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	Biochem. Biophys. Res. Commun. Aug (2003); 308(1):170-6
CHD1 associates with NCoR and histone deacetylase as well as with RNA splicing proteins.
Tai HH, Geisterfer M, Bell JC, Moniwa M, Davie JR, Boucher L, McBurney MW
Ottawa Regional Cancer Centre, and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ont., Canada. htai@nrcan.gc.ca
Abstract: CHD1 is one of a family of nuclear proteins containing two chromodomains, a SWI/SNF-like helicase/ATPase domain and a DNA binding domain. We found that CHD1 co-immunoprecipitates with histone deacetylase (HDAC) activity and that CHD1 also associates with NCoR, a transcriptional corepressor, in yeast two-hybrid and in vitro pull-down assays. NCoR is known to associate with HDACs to effect its repressive activity, suggesting that the predicted chromatin remodeling activity of CHD1 plays a role in this repression. Yeast two-hybrid assays also showed that CHD1 interacts with splicing proteins mKIAA0164, Srp20, and SAF-B. Splicing assays show that CHD1 overexpression can affect alternative splicing. These results suggest that CHD1 may function in both chromatin mediated transcriptional repression and RNA splicing.
[PUBMED: 12890497]