The BioGRID | PUBLICATION VIEW

	Proc. Natl. Acad. Sci. U.S.A. Sep (1992); 89(18):8769-73
Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.
Zhang M, Zamore PD, Carmo-Fonseca M, Lamond AI, Green MR
Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605.
Abstract: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.
[PUBMED: 1388271]