The BioGRID | PUBLICATION VIEW

	PLoS ONE (2008); 3(5):e2223
The yeast Tor signaling pathway is involved in G2-M transition via polo-kinase-
Nakashima A, Maruki Y, Imamura Y, Kondo C, Kawamata T, Kawanishi I, Takata H, Matsuura A, Lee KS, Kikkawa U, Ohsumi Y, Yonezawa K, Kamada Y
Biosignal Research Center, Kobe University, Kobe, Japan-
Abstract: The target of rapamycin -Tor- protein plays central roles in cell growth- Rapamycin inhibits cell growth and promotes cell cycle arrest at G1 -G0-- However, little is known about whether Tor is involved in other stages of the cell division cycle- Here we report that the rapamycin-sensitive Tor complex 1 -TORC1- is involved in G2-M transition in S- cerevisiae- Strains carrying a temperature-sensitive allele of KOG1 -kog1-105- encoding an essential component of TORC1, as well as yeast cell treated with rapamycin show mitotic delay with prolonged G2- Overexpression of Cdc5, the yeast polo-like kinase, rescues the growth defect of kog1-105, and in turn, Cdc5 activity is attenuated in kog1-105 cells- The TORC1-Type2A phosphatase pathway mediates nucleocytoplasmic transport of Cdc5, which is prerequisite for its proper localization and function- The C-terminal polo-box domain of Cdc5 has an inhibitory role in nuclear translocation- Taken together, our results indicate a novel function of Tor in the regulation of cell cycle and proliferation-
[PUBMED: 18493323]