Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us Genes Dev. Jan (2007); 21(2):148-59 Modeling complex genetic interactions in a simple eukaryotic genome- actin displays a rich spectrum of complex haploinsufficiencies- Haarer B, Viggiano S, Hibbs MA, Troyanskaya OG, Amberg DC Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University, Syracuse, New York 13210, USA- Abstract: Multigenic influences are major contributors to human genetic disorders- Since humans are highly polymorphic, there are a high number of possible detrimental, multiallelic gene pairs- The actin cytoskeleton of yeast was used to determine the potential for deleterious bigenic interactions; approximately 4800 complex hemizygote strains were constructed between an actin-null allele and the nonessential gene deletion collection- We found 208 genes that have deleterious complex haploinsufficient -CHI- interactions with actin- This set is enriched for genes with gene ontology terms shared with actin, including several actin-binding protein genes, and nearly half of the CHI genes have defects in actin organization when deleted- Interactions were frequently seen with genes for multiple components of a complex or with genes involved in the same function- For example, many of the genes for the large ribosomal subunit -RPLs- were CHI with act1Delta and had actin organization defects when deleted- This was generally true of only one RPL paralog of apparently duplicate genes, suggesting functional specialization between ribosomal genes- In many cases, CHI interactions could be attributed to localized defects on the actin protein- Spatial congruence in these data suggest that the loss of binding to specific actin-binding proteins causes subsets of CHI interactions- [PUBMED: 17167106] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.