The BioGRID | PUBLICATION VIEW

	Mol. Cell Feb (2008); 29(4):517-24
Rad52 promotes postinvasion steps of meiotic double-strand-break repair-
Lao JP, Oh SD, Shinohara M, Shinohara A, Hunter N
Section of Microbiology, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA-
Abstract: During DNA double-strand-break -DSB- repair by recombination, the broken chromosome uses a homologous chromosome as a repair template- Early steps of recombination are well characterized- DSB ends assemble filaments of RecA-family proteins that catalyze homologous pairing and strand-invasion reactions- By contrast, the postinvasion steps of recombination are poorly characterized- Rad52 plays an essential role during early steps of recombination by mediating assembly of a RecA homolog, Rad51, into nucleoprotein filaments- The meiosis-specific RecA-homolog Dmc1 does not show this dependence, however- By exploiting the Rad52 independence of Dmc1, we reveal that Rad52 promotes postinvasion steps of both crossover and noncrossover pathways of meiotic recombination in Saccharomyces cerevisiae- This activity resides in the N-terminal region of Rad52, which can anneal complementary DNA strands, and is independent of its Rad51-assembly function- Our findings show that Rad52 functions in temporally and biochemically distinct reactions and suggest a general annealing mechanism for reuniting DSB ends during recombination-
[PUBMED: 18313389]