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| Genetics Feb (2008); 178(2):649-59 | |
| A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae- | |
| Biswas D, Takahata S, Xin H, Dutta-Biswas R, Yu Y, Formosa T, Stillman DJ | |
| Department of Pathology, University of Utah Health Sciences Center, Salt Lake City, Utah 84112, USA- | |
| Abstract: Chromatin-modifying factors regulate both transcription and DNA replication- The yFACT chromatin-reorganizing complex is involved in both processes, and the sensitivity of some yFACT mutants to the replication inhibitor hydroxyurea -HU- is one indication of a replication role- This HU sensitivity can be suppressed by disruptions of the SET2 or CHD1 genes, encoding a histone H3-K36- methyltransferase and a chromatin remodeling factor, respectively- The additive effect of set2 and chd1 mutations in suppressing the HU sensitivity of yFACT mutants suggests that these two factors function in separate pathways- The HU suppression is not an indirect effect of altered regulation of ribonucleotide reductase induced by HU- set2 and chd1 mutations also suppress the HU sensitivity of mutations in other genes involved in DNA replication, including CDC2, CTF4, ORC2, and MEC1- Additionally, a chd1 mutation can suppress the lethality normally caused by disruption of either MEC1 or RAD53 DNA damage checkpoint genes, as well as the lethality seen when a mec1 sml1 mutant is exposed to low levels of HU- The pob3 defect in S-phase progression is suppressed by set2 or chd1 mutations, suggesting that Set2 and Chd1 have specific roles in negatively regulating DNA replication- | |
| [PUBMED: 18245327] |   |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |