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	Arch. Biochem. Biophys. Jan (2008); 469(2):157-64
Interacting proteins Rtt109 and Vps75 affect the efficiency of non-homologous end-joining in Saccharomyces cerevisiae-
Jessulat M, Alamgir M, Salsali H, Greenblatt J, Xu J, Golshani A
Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ont-, Canada K1S 5B6-
Abstract: One of the key pathways for DNA double-stranded break -DSB- repair is the non-homologous end-joining -NHEJ- pathway, which directly re-ligates two broken ends of DNA- Using a plasmid repair assay screen, we identified that the deletion strain for RTT109 had a reduced efficiency for NHEJ in yeast- This deletion strain also had a reduced efficiency to repair induced chromosomal DSBs in vivo- Tandem-affinity purification of Rtt109 recovered Vps75 as a physical interacting protein- Deletion of VPS75 was also shown to have an effect on the efficiency of NHEJ in both the plasmid repair and the chromosomal repair assays- In addition, deletion mutants for both RTT109 and VPS75 showed hypersensitivity to different DNA damaging agents- Our genetic interaction analysis supports a role for RTT109 in DNA damage repair- We propose that one function of the Rtt109-Vps75 interacting protein pair is to affect the efficiency of NHEJ in yeast- Vps75 but not Rtt109 also seem to have an effect on the efficiency of DSB repair using homologous recombination-
[PUBMED: 18036332]