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| Proc. Natl. Acad. Sci. U.S.A. Apr (2008); 105(15):5821-6 | |
| Linking functionally related genes by sensitive and quantitative characterization of genetic interaction profiles- | |
| Decourty L, Saveanu C, Zemam K, Hantraye F, Frachon E, Rousselle JC, Fromont-Racine M, Jacquier A | |
| Institut Pasteur, Genetique des Interactions Macromoleculaires, Centre National de la Recherche Scientifique, Unite de Recherche Associee 2171, Paris Cedex 15, France- | |
| Abstract: Describing at a genomic scale how mutations in different genes influence one another is essential to the understanding of how genotype correlates with phenotype and remains a major challenge in biology- Previous studies pointed out the need for accurate measurements of not only synthetic but also buffering interactions in the characterization of genetic networks and functional modules- We developed a sensitive and efficient method that allows such measurements at a genomic scale in yeast- In a pilot experiment -41 genome-wide screens-, we quantified the fitness of 140,000 double deletion strains relative to the corresponding single mutants and identified many genetic interactions- In addition to synthetic growth defects -validated experimentally with factors newly identified as genetically interfering with mRNA degradation-, most of the identified genetic interactions measured weak epistatic effects- These weak effects, rarely meaningful when considered individually, were crucial to defining specific signatures for many gene deletions and had a major contribution in defining clusters of functionally related genes- | |
| [PUBMED: 18408161] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |