Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us PLoS ONE (2008); 3(2):e1617 Ybp2 Associates with the Central Kinetochore of Saccharomyces cerevisiae and Mediates Proper Mitotic Progression- Ohkuni K, Abdulle R, Tong AH, Boone C, Kitagawa K Department of Molecular Pharmacology, St- Jude Children's Research Hospital, Memphis, Tennessee, United States of America- Abstract: The spindle checkpoint ensures the accurate segregation of chromosomes by monitoring the status of kinetochore attachment to microtubules- Simultaneous mutations in one of several kinetochore and cohesion genes and a spindle checkpoint gene cause a synthetic-lethal or synthetic-sick phenotype- A synthetic genetic array -SGA- analysis using a mad2Delta query mutant strain of yeast identified YBP2, a gene whose product shares sequence similarity with the product of YBP1, which is required for H-2-O-2--induced oxidation of the transcription factor Yap1- ybp2Delta was sensitive to benomyl and accumulated at the mitotic stage of the cell cycle- Ybp2 physically associates with proteins of the COMA complex -Ctf19, Okp1, Mcm21, and Ame1- and 3 components of the Ndc80 complex -Ndc80, Nuf2, and Spc25 but not Spc24- in the central kinetochore and with Cse4 -the centromeric histone and CENP-A homolog-- Chromatin-immunoprecipitation analyses revealed that Ybp2 associates specifically with CEN DNA- Furthermore, ybp2Delta showed synthetic-sick interactions with mutants of the genes that encode the COMA complex components- Ybp2 seems to be part of a macromolecular kinetochore complex and appears to contribute to the proper associations among the central kinetochore subcomplexes and the kinetochore-specific nucleosome- [PUBMED: 18286174] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.