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	J. Microbiol. Methods Mar (2008); 72(3):306-12
Engineered bakers yeast as a sensitive bioassay indicator organism for the trichothecene toxin deoxynivalenol-
Abolmaali S, Mitterbauer R, Spadiut O, Peruci M, Weindorfer H, Lucyshyn D, Ellersdorfer G, Lemmens M, Moll WD, Adam G
Center of Applied Genetics and Cell Biology, BOKU - University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190 Vienna, Austria-
Abstract: The aim of this study was to increase the sensitivity of Saccharomyces cerevisiae towards trichothecene toxins, in particular to deoxynivalenol -DON-, in order to improve the utility of this yeast as a bioassay indicator organism- We report the construction of a strain with inactivated genes -PDR5, PDR10, PDR15- encoding ABC transporter proteins with specificity for the trichothecene deoxynivalenol, with inactivated AYT1 -encoding a trichothecene-3-O-acetyltransferase-, and inactivated UBI4 and UBP6 genes- Inactivation of the stress inducible polyubiquitin gene UBI4 or the ubiquitin protease UBP6 increased DON sensitivity, the inactivation of both genes had a synergistic effect- The resulting pdr5 pdr10 pdr15 ayt1 ubp6 ubi4 mutant strain showed 50% growth inhibition at a DON concentration of 5 mg-l under optimal conditions- The development of a simple two step assay for microbial DON degradation in 96 well microtiter format and its testing with the DON detoxifying bacterium BBSH 797 is reported-
[PUBMED: 18243380]