|
|
| J. Cell Biol. Feb (2008); 180(3):579-95 | |
| The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery- | |
| Boulon S, Marmier-Gourrier N, Pradet-Balade B, Wurth L, Verheggen C, Jady BE, Rothe B, Pescia C, Robert MC, Kiss T, Bardoni B, Krol A, Branlant C, Allmang C, Bertrand E, Charpentier B | |
| Institute of Molecular Genetics of Montpellier, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 5535, Montpellier Cedex 5, France- | |
| Abstract: RNA-binding proteins of the L7Ae family are at the heart of many essential ribonucleoproteins -RNPs-, including box C-D and H-ACA small nucleolar RNPs, U4 small nuclear RNP, telomerase, and messenger RNPs coding for selenoproteins- In this study, we show that Nufip and its yeast homologue Rsa1 are key components of the machinery that assembles these RNPs- We observed that Rsa1 and Nufip bind several L7Ae proteins and tether them to other core proteins in the immature particles- Surprisingly, Rsa1 and Nufip also link assembling RNPs with the AAA - adenosine triphosphatases hRvb1 and hRvb2 and with the Hsp90 chaperone through two conserved adaptors, Tah1-hSpagh and Pih1- Inhibition of Hsp90 in human cells prevents the accumulation of U3, U4, and telomerase RNAs and decreases the levels of newly synthesized hNop58, hNHP2, 15-5K, and SBP2- Thus, Hsp90 may control the folding of these proteins during the formation of new RNPs- This suggests that Hsp90 functions as a master regulator of cell proliferation by allowing simultaneous control of cell signaling and cell growth- | |
| [PUBMED: 18268104] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
| terms and conditions - privacy policy - Osprey Network Visualization System |
| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |