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| Mol. Cell Proteomics Nov (2007); 6(11):1968-79 | |
| The Saccharomyces homolog of mammalian RACK1, Cpc2-Asc1p, is required for FLO11-dependent adhesive growth and dimorphism- | |
| Valerius O, Kleinschmidt M, Rachfall N, Schulze F, Lopez Marin S, Hoppert M, Streckfuss-Bomeke K, Fischer C, Braus GH | |
| Institute of Microbiology and Genetics, Georg August University, D-37077 Gottingen, Germany- | |
| Abstract: Nutrient starvation results in the interaction of Saccharomyces cerevisiae cells with each other and with surfaces- Adhesive growth requires the expression of the FLO11 gene regulated by the Ras-cAMP-cAMP-dependent protein kinase, the Kss1p-MAPK, and the Gcn4p-general amino acid control pathway, respectively- Proteomics two-dimensional DIGE experiments revealed post-transcriptionally regulated proteins in response to amino acid starvation including the ribosomal protein Cpc2p-Asc1p- This putative translational regulator is highly conserved throughout the eukaryotic kingdom and orthologous to mammalian RACK1- Deletion of CPC2-ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its activation upon starvation in haploid cells- In addition, the diploid Flo11p-dependent pseudohyphal growth during nitrogen limitation was CPC2-ASC1-dependent- A more detailed analysis revealed that a CPC2-ASC1 deletion caused increased sensitivity to cell wall drugs suggesting that the gene is required for general cell wall integrity- Phosphoproteome and Western hybridization data indicate that Cpc2p-Asc1p affected the phosphorylation of the translational initiation factors eIF2 alpha and eIF4A and the ribosome-associated complex RAC- A crucial role of Cpc2p-Asc1p at the ribosomal interface coordinating signal transduction, translation initiation, and transcription factor formation was corroborated- | |
| [PUBMED: 17704055] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |