The BioGRID | PUBLICATION VIEW

	Eukaryotic Cell Dec (2007); 6(12):2251-9
The Ccr4-Not Complex Regulates Skn7 through Srb10 Kinase-
Lenssen E, Azzouz N, Michel A, Landrieux E, Collart MA
Department of Microbiology and Molecular Medicine, University of Geneva Medical School, CMU, 1 rue Michel Servet, 1211 Geneva 4, Switzerland- martine-collart@medecine-unige-ch-
Abstract: The Ccr4-Not complex is a multifunctional regulatory platform composed of nine subunits that controls diverse cellular events including mRNA degradation, protein ubiquitination, and transcription- In this study, we identified the yeast Saccharomyces cerevisiae osmotic and oxidative stress transcription factor Skn7 as a new target for regulation by the Ccr4-Not complex- Skn7 interacts with Not1 in a two-hybrid assay and coimmunoprecipitates with Not5 in a Not4-dependent manner- Skn7-dependent expression of OCH1 and Skn7 binding to the OCH1 promoter are increased in not4Delta or not5Delta mutants- Skn7 purified from wild-type cells but not from not4Delta cells is associated with the Srb10 kinase- This kinase plays a central role in the regulation of Skn7 by Not4, since increased OCH1 expression in not4Delta cells requires Srb10- These results reveal a critical role for the Ccr4-Not complex in the mechanism of activation of Skn7 that is dependent upon the Srb10 kinase-
[PUBMED: 17965252]