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| Nucleic Acids Res. (2007); 35(18):6017-28 | |
| A specific role for the C-terminal region of the Poly-A--binding protein in mRNA decay- | |
| Simon E, Seraphin B | |
| Equipe Labellisee La Ligue, Centre de Genetique Moleculaire, CNRS UPR2167 associee a l'Universite P- et M- Curie, Avenue de la Terrasse, 91198 Gif sur Yvette, France- | |
| Abstract: mRNA poly-A- tails affect translation, mRNA export and mRNA stability, with translation initiation involving a direct interaction between eIF4G and the poly-A--binding protein Pab1- The latter factor contains four RNA recognition motifs followed by a C-terminal region composed of a linker and a PABC domain- We show here that yeast mutants lacking the C-terminal domains of Pab1 display specific synthetic interactions with mutants in the 5'-3' mRNA decay pathway- Moreover, these mutations impair mRNA decay in vivo without significantly affecting mRNA export or translation- Inhibition of mRNA decay occurs through slowed deadenylation- In vitro analyses demonstrate that removal of the Pab1 linker domain directly interferes with the ability of the Pop2-Ccr4 complex to deadenylate the Pab1-bound poly-A-- Binding assays demonstrate that this results from a modulation of poly-A- packaging by the Pab1 linker region- Overall, our results demonstrate a direct involvement of Pab1 in mRNA decay and reveal the modular nature of this factor, with different domains affecting various cellular processes- These data suggest new models involving the modulation of poly-A- packaging by Pab1 to control mRNA decay- | |
| [PUBMED: 17766253] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |