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| Genetics Nov (2007); 177(3):1679-89 | |
| Flo11p-independent control of "mat" formation by hsp70 molecular chaperones and nucleotide exchange factors in yeast- | |
| Martineau CN, Beckerich JM, Kabani M | |
| Laboratoire d'Enzymologie et Biochimie Structurales -LEBS-, CNRS 91198 Gif-sur-Yvette Cedex, France- | |
| Abstract: The yeast Saccharomyces cerevisiae has been used as a model for fungal biofilm formation due to its ability to adhere to plastic surfaces and to form mats on low-density agar petri plates- Mats are complex multicellular structures composed of a network of cables that form a central hub from which emanate multiple radial spokes- This reproducible and elaborate pattern is indicative of a highly regulated developmental program that depends on specific transcriptional programming, environmental cues, and possibly cell-cell communication systems- While biofilm formation and sliding motility were shown to be strictly dependent on the cell-surface adhesin Flo11p, little is known about the cellular machinery that controls mat formation- Here we show that Hsp70 molecular chaperones play key roles in this process with the assistance of the nucleotide exchange factors Fes1p and Sse1p and the Hsp40 family member Ydj1p- The disruption of these cofactors completely abolished mat formation- Furthermore, complex interactions among SSA genes were observed- mat formation depended mostly on SSA1 while minor defects were observed upon loss of SSA2; additional mutations in SSA3 or SSA4 further enhanced these phenotypes- Importantly, these mutations did not compromise invasive growth or Flo11p expression, suggesting that Flo11p-independent pathways are necessary to form mats- | |
| [PUBMED: 17947402] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |