The BioGRID | PUBLICATION VIEW

	Mol. Biol. Cell Oct (2007); 18(10):3800-9
A protein interaction map of the mitotic spindle-
Wong J, Nakajima Y, Westermann S, Shang C, Kang JS, Goodner C, Houshmand P, Fields S, Chan CS, Drubin D, Barnes G, Hazbun T
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA-
Abstract: The mitotic spindle consists of a complex network of proteins that segregates chromosomes in eukaryotes- To strengthen our understanding of the molecular composition, organization, and regulation of the mitotic spindle, we performed a system-wide two-hybrid screen on 94 proteins implicated in spindle function in Saccharomyces cerevisiae- We report 604 predominantly novel interactions that were detected in multiple screens, involving 303 distinct prey proteins- We uncovered a pattern of extensive interactions between spindle proteins reflecting the intricate organization of the spindle- Furthermore, we observed novel connections between kinetochore complexes and chromatin-modifying proteins and used phosphorylation site mutants of NDC80-TID3 to gain insights into possible phospho-regulation mechanisms- We also present analyses of She1p, a novel spindle protein that interacts with the Dam1 kinetochore-spindle complex- The wealth of protein interactions presented here highlights the extent to which mitotic spindle protein functions and regulation are integrated with each other and with other cellular activities-
[PUBMED: 17634282]