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Genes Dev. Sep (2004); 18(18):2255-68
A conserved protein network controls assembly of the outer kinetochore and its ability to sustain tension-
Cheeseman IM, Niessen S, Anderson S, Hyndman F, Yates JR, Oegema K, Desai A
Ludwig Institute for Cancer Research, La Jolla, California 92093, USA- icheeseman@ucsd-edu
Abstract: Kinetochores play an essential role in chromosome segregation by forming dynamic connections with spindle microtubules- Here, we identify a set of 10 copurifying kinetochore proteins from Caenorhabditis elegans, seven of which were previously uncharacterized- Using in vivo assays to monitor chromosome segregation, kinetochore assembly, and the mechanical stability of chromosome-microtubule attachments, we show that this copurifying protein network plays a central role at the kinetochore-microtubule interface- In addition, our analysis suggests that the network is comprised of three groups of proteins that contribute in distinct ways to this interface- KNL proteins act after the assembly of centromeric chromatin to generate the core of the microtubule-binding interface, MIS proteins control the rate and extent of formation of this interface, and NDC proteins are necessary to sustain tension during interactions with spindle microtubules- We also purify a similar set of associated proteins from human cells that includes four novel proteins and has recognizable homologs from each functional class- Thus, this protein network is a conserved constituent of the outer kinetochore, and the functions defined by our analysis in C- elegans are likely to be widely relevant-
[PUBMED: 15371340] Download Biogrid Interactions in a variety of formats including PSI FormatPUBMED
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Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers.
Nucleic Acids Res. Jan 1;34:D535-9.