The BioGRID | PUBLICATION VIEW

	Jul (2007); 0:
Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae-
Devasahayam G, Burke DJ, Sturgill TW
University of Virginia Health Sciences Center-
Abstract: TThe Pmr1 Golgi Ca2--Mn2- ATPase negatively regulates TORC1 signaling, the rapamycin sensitive TOR complex in Saccharomyces cerevisiae- Since pmr1 causes resistance to rapamycin and tor1 causes hypersensitivity, we looked for genetic interactions of pmr1 with tor1- Deletion of TOR1 restored two wild type phenotypes- Loss of TOR1 restored ability of the pmr1 strain to grow on media containing 2 mM Mn2-Cl2 like wild type as well as the wild type sensitivity to rapamycin- Mn2- additions to media partially suppressed rapamycin resistance of wild type and pmr1 tor1, suggesting that Tor1 and Tor2 are regulated by manganese- We parsed the roles of Ca2- and Mn2- transport and the compartments in rapamycin response using separation-of-function mutants available for Pmr1- A strain containing D53A mutant -Mn2- transporting- of Pmr1 is rapamycin sensitive, but the Q783A mutant -Ca2- transporting- strain is rapamycin resistant- Mn2- transport into the Golgi lumen appears to be required for rapamycin sensitivity- Overexpression of Ca2- pump SERCA1, Ca2-- H- antiporter Vcx1, or a Mn2- transporting mutant of Vcx1 -Vcx1-M1- failed to restore rapamycin sensitivity, and loss of Pmr1 but not other transporters of Ca2- or Mn2- results in rapamycin resistance- Overexpression of Ccc1, a Fe2- and Mn2- transporter that has been localized to Golgi and the vacuole does restore rapamycin sensitivity to pmr1- We conclude that Mn2- in the Golgi inhibits TORC1 signaling-
[PUBMED: 17603109]

terms and conditions - privacy policy - Osprey Network Visualization System

BioGRID: A General Repository for Interaction Datasets.
Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers.
Nucleic Acids Res. Jan 1;34:D535-9.