Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us Proc. Natl. Acad. Sci. U.S.A. Apr (2007); 104(14):5836-41 CCR4-NOT complex associates with the proteasome and regulates histone methylation- Laribee RN, Shibata Y, Mersman DP, Collins SR, Kemmeren P, Roguev A, Weissman JS, Briggs SD, Krogan NJ, Strahl BD Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA- Abstract: The proteasome regulates histone lysine methylation and gene transcription, but how it does so is poorly understood- To better understand this process, we used the epistatic miniarray profile -E-MAP- approach to identify factors that genetically interact with proteasomal subunits- In addition to members of the Set1 complex that mediate histone H3 lysine 4 methylation -H3K4me-, we found that deleting members of the CCR4-NOT mRNA processing complex exhibit synthetic phenotypes when combined with proteasome mutants- Further biochemical analyses revealed physical associations between CCR4-NOT and the proteasome in vivo- Consistent with the genetic and biochemical interactions linking CCR4-NOT with proteasome and Set1-mediated methylation, we find that loss of Not4 decreases global and gene-specific H3K4 trimethylation -H3K4me3- and decreases 19S proteasome recruitment to the PMA1 gene- Similar to proteasome regulation of histone methylation, loss of CCR4-NOT members does not affect ubiquitinated H2B- Mapping of Not4 identified the RING finger domain as essential for H3K4me3, suggesting a role for ubiquitin in this process- Consistent with this idea, loss of the Not4-interacting protein Ubc4, a known ubiquitin-conjugating enzyme, decreases H3K4me3- These studies implicate CCR4-NOT in the regulation of H3K4me3 through a ubiquitin-dependent pathway that likely involves the proteasome- [PUBMED: 17389396] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.