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| Jul (2007); 0: | |
| Stimulation of in vitro sumoylation by Slx5-Slx8- Evidence for a functional interaction with the SUMO pathway- | |
| Ii T, Mullen JR, Slagle CE, Brill SJ | |
| Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08854, USA- | |
| Abstract: The yeast genes SLX5 and SLX8 were identified based on their requirement for viability in the absence of the Sgs1 DNA helicase- Loss of these genes results in genome instability, nibbled colonies, and other phenotypes associated with defects in sumoylation- The Slx5 and Slx8 proteins form a stable complex and each subunit contains a single RING-finger domain at its C-terminus- To determine the physiological function of the Slx5-8 complex, we explored its interaction with the SUMO pathway- Curing 2mu circle from the mutants, suppressed their nibbled colony phenotype and partially improved their growth rate, but did not affect their sensitivity to hydroxyurea- The increase in sumoylation observed in slx5Delta and slx8Delta mutants was found to be dependent on the Siz1 SUMO ligase- Physical interactions between the Slx5-8 complex and both Ubc9 and Smt3 were identified and characterized- Using in vitro reactions, we show that Slx5, Slx8, or the Slx5-8 complex stimulates the formation of SUMO chains and the sumoylation of a test substrate- Interestingly, a functional RING-finger domain is not required for this stimulation in vitro- These biochemical data demonstrate for the first time that the Slx5 and Slx8 complex is capable of interacting directly with the SUMO pathway- | |
| [PUBMED: 17669696] |   |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |