Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us Mol. Cell Proteomics May (2006); 5(5):824-34 Physical and genetic interactions link hox function with diverse transcription factors and cell signaling proteins- Bondos SE, Tan XX, Matthews KS Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA- bondos@rice-edu Abstract: Positional information provided by Hox homeotic transcription factors is integrated with other transcription factors and cell signaling cascades in specific combinations to dictate context- and gene-specific Hox activity- Protein-protein interactions between these groups have long been hypothesized to modulate Hox functions, yielding a context-specific function- However, difficulties in applying interaction screens to potent transcription factors have limited partner identification- A yeast two-hybrid screen using transcription activation-deficient mutants of the Drosophila melanogaster Hox protein Ultrabithorax IB identified an array of interacting proteins, consisting primarily of transcription factors and components of cell signaling pathways- Interactions were confirmed with wild-type Ultrabithorax -UBX- in phage display experiments and by immunoprecipitation for a subset of partners- In vivo assays demonstrated that two Ultrabithorax IB partners, Armadillo, regulated by Wingless-WNT signaling, and the homeodomain protein Aristaless, inhibit UBX-dependent haltere development from the default wing development pathway- Therefore, transcription factors and cell signaling proteins that subdivide Hox-specified tissues can both alter Hox function in vivo and interact with the corresponding Hox protein in vitro- UBX may also modulate partner function- the pupal death phenotype induced by ectopic expression of the UBX partner Hairy required the presence of UBX- Thus, Hox-transcription factor complexes may integrate a variety of positional cues, generating the specificity and versatility required for context-dependent Hox function- [PUBMED: 16455680] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.