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| Yeast Apr (2007); 24(4):239-52 | |
| Dissecting the role of dolichol in cell wall assembly in the yeast mutants impaired in early glycosylation reactions- | |
| Or?owski J, Machula K, Janik A, Zdebska E, Palamarczyk G | |
| Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, Warsaw, Poland- | |
| Abstract: Evidence is presented that temperature-sensitive Saccharomyces cerevisiae mutants, impaired in dolichol kinase -Sec59p- or dolichyl phosphate mannose synthase -Dpm1p- activity have an aberrant cell wall composition and ultrastructure- The mutants were oversensitive to Calcofluor white, an agent interacting with the cell wall chitin- In accordance with this, chemical analysis of the cell wall alkali-insoluble fraction indicated an increased amount of chitin and changes in the quantity of beta1,6- and beta1,3-glucan in sec59-1 and dpm1-6 mutants- In order to unravel the link between the formation of dolichyl phosphate and dolichyl phosphate mannose and the cell wall assembly, we screened a yeast genomic library for a multicopy suppressors of the thermosensitive phenotype- The RER2 and SRT1 genes, encoding cis-prenyltransferases, were isolated- In addition, the ROT1 gene, encoding protein involved in beta1,6-glucan synthesis -Machi et al-, 2004- and protein folding -Takeuchi et al-, 2006- acted as a multicopy suppressor of the temperature-sensitive phenotype of the sec59-1 mutant- The cell wall of the mutants and of mutants bearing the multicopy suppressors was analysed for carbohydrate and mannoprotein content- We also examined the glycosylation status of the plasma membrane protein Gas1p, a beta1,3-glucan elongase, and the degree of phosphorylation of the Mpk1-Slt2 protein, involved in the cell wall integrity pathway- | |
| [PUBMED: 17397129] |   |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |