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| Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. Mar (2007); 63:190-2 | |
| Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae- | |
| Nakamura Y, Umehara T, Tanaka A, Horikoshi M, Padmanabhan B, Yokoyama S | |
| RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan- | |
| Abstract: The non-ATPase subunit Nas6, which is the human orthologue of gankyrin, was co-expressed with the C-terminal domain of the ATPase subunit Rpt3 of the yeast 26S proteasome in Escherichia coli, purified to near-homogeneity and crystallized using the hanging-drop vapour-diffusion method- The protein crystallized in space group P2-1-, with unit-cell parameters a = 60-38, b = 100-22, c = 72-20 A, beta = 94-70 degrees and with three Nas6-Rpt3C molecules per asymmetric unit- The crystal diffracted to beyond 2-2 A resolution using synchrotron radiation- | |
| [PUBMED: 17329811] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |