The BioGRID | PUBLICATION VIEW

	Science Feb (2007); 315(5812):646-9
The DEAD-box RNA helicase Dbp5 functions in translation termination-
Gross T, Siepmann A, Sturm D, Windgassen M, Scarcelli JJ, Seedorf M, Cole CN, Krebber H
Institut fur Molekularbiologie und Tumorforschung der Philipps-Universitat Marburg, Emil-Mannkopff-Strasse 2, 35037 Marburg, Germany-
Abstract: In eukaryotes, termination of messenger RNA -mRNA- translation is mediated by the release factors eRF1 and eRF3- Using Saccharomyces cerevisiae as a model organism, we have identified a member of the DEAD-box protein -DBP- family, the DEAD-box RNA helicase and mRNA export factor Dbp5, as a player in translation termination- Dbp5 interacts genetically with both release factors and the polyadenlyate-binding protein Pab1- A physical interaction was specifically detected with eRF1- Moreover, we show that the helicase activity of Dbp5 is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes- Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events-
[PUBMED: 17272721]