The BioGRID | PUBLICATION VIEW

	Traffic Oct (2006); 7(10):1368-77
Vac8p, an armadillo repeat protein, coordinates vacuole inheritance with multiple vacuolar processes-
Tang F, Peng Y, Nau JJ, Kauffman EJ, Weisman LS
Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA- fxtang@ualr-edu
Abstract: Vac8p, an armadillo -ARM- repeat protein, is required for multiple vacuolar processes- It functions in vacuole inheritance, cytoplasm-to-vacuole protein targeting pathway, formation of the nucleus-vacuole junction and vacuole-vacuole fusion- These functions each utilize a distinct Vac8p-binding partner- Here, we report an additional Vac8p function- caffeine resistance- We show that Vac8p function in caffeine resistance is mediated via a newly identified Vac8p-binding partner, Tco89p- The interaction between Vac8p and each binding partner requires an overlapping subset of Vac8p ARM repeats- Moreover, these partners can compete with each other for access to Vac8p- Furthermore, Vac8p is enriched in three separate subdomains on the vacuole, each with a unique binding partner dedicated to a different vacuolar function- These findings suggest that a major role of Vac8p is to spatially separate multiple functions thereby enabling vacuole inheritance to occur concurrently with other vacuolar processes-
[PUBMED: 16824055]