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	Mol. Biol. Cell Dec (2005); 16(12):5649-60
De novo kinetochore assembly requires the centromeric histone H3 variant-
Collins KA, Castillo AR, Tatsutani SY, Biggins S
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1042, USA-
Abstract: Kinetochores mediate chromosome attachment to the mitotic spindle to ensure accurate chromosome segregation- Budding yeast is an excellent organism for kinetochore assembly studies because it has a simple defined centromere sequence responsible for the localization of >65 proteins- In addition, yeast is the only organism where a conditional centromere is available to allow studies of de novo kinetochore assembly- Using a conditional centromere, we found that yeast kinetochore assembly is not temporally restricted and can occur in both G1 phase and prometaphase- We performed the first investigation of kinetochore assembly in the absence of the centromeric histone H3 variant Cse4 and found that all proteins tested depend on Cse4 to localize- Consistent with this observation, Cse4-depleted cells had severe chromosome segregation defects- We therefore propose that yeast kinetochore assembly requires both centromeric DNA specificity and centromeric chromatin-
[PUBMED: 16207811]