Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us (2006); 1:28 Yeast UBL-UBA proteins have partially redundant functions in cell cycle control- Diaz-Martinez LA, Kang Y, Walters KJ, Clarke DJ Department of Genetics, Cell Biology and Development, University of Minnesota, 6-160 Jackson Hall, 321 Church Street SE, Minneapolis, USA- clark140@umn-edu- Abstract: ABSTRACT- BACKGROUND- Proteins containing ubiquitin-like -UBL- and ubiquitin associated -UBA- domains have been suggested to shuttle ubiquitinated substrates to the proteasome for degradation- There are three UBL-UBA containing proteins in budding yeast- Ddi1, Dsk2 and Rad23, which have been demonstrated to play regulatory roles in targeting ubiquitinated substrates to the proteasome for degradation- An involvement of these proteins in cell cycle related events has also been reported- We tested whether these three proteins act redundantly in the cell cycle- RESULTS- Here we show that the UBL-UBA proteins are partially redundant for cell cycle related roles- RAD23 is redundant with DDI1 and DSK2, but DDI1 and DSK2 are not redundant with each other and the triple deletion shows a synthetic effect, suggesting the existence of at least two roles for RAD23 in cell cycle control- The rad23Deltaddi1Deltadsk2Delta triple deletion strain delays both in G2-M-phase and in mid-anaphase at high temperatures with duplicated spindle pole bodies- Cell cycle progression in the triple deletion strain can only be partially rescued by a rad23 allele lacking the c-terminal UBA domain, suggesting that RAD23 requires its c-terminal UBA domain for full function- In addition to their ability to bind ubiquitin and the proteasome, the UBL-UBA proteins also share the ability to homodimerize- Rad23 and Dsk2 dimerization requires their UBL and-or UBA domains whereas Ddi1 dimerization does not- Here we show that Ddi1 homodimerization is necessary for its cell cycle related functions- CONCLUSION- The three yeast UBL-UBA proteins have partially redundant roles required for progression through mitosis- [PUBMED: 17144915] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.