Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us J. Mol. Biol. Sep (2005); 352(2):355-69 Deletion of EFL1 results in heterogeneity of the 60 S GTPase-associated rRNA conformation- Graindorge JS, Rousselle JC, Senger B, Lenormand P, Namane A, Lacroute F, Fasiolo F UPR no- 9002 du CNRS, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15, rue Rene Descartes, 67084 Strasbourg Cedex, France- Abstract: Previous work suggested that the release of the nucleolar Tif6 from nascent 60 S subunits occurs in the cytoplasm and requires the cytoplasmic EF-2-like GTPase, Efl1- To check whether this release involves an rRNA structural rearrangement mediated by Efl1, we analyzed the rRNA conformation of the GTPase center of 80 S ribosomes in three contexts- wild-type, Deltaefl1 and a dominant suppressor R1 of Deltaefl1- This analysis was restricted to domain II and VI of 25 S rRNA- The rRNA analysis of R1 ribosomes allows us to distinguish the effects due to depletion of Efl1 from the resulting nucleolar deficit of Tif6- Efl1 inhibits the EF-2 GTPase activity, suggesting that the two proteins share a similar ribosome-binding site- The 80 S ribosomes from either type failed to show any difference of conformation in the two rRNA domains analyzed- However, the same analysis performed on the pool of free 60 S subunits reveals several rRNA conformational differences between wild-type and Deltaefl1 subunits, whereas that from the suppressor strain is similar to wild-type- This suggests that the nucleolar deficit of Tif6 during assembly of the 60 S preribosomes is responsible for the changes in rRNA conformation observed in Deltaefl1 60 S subunits- We also purified 60 S preribosomes from the three genetic contexts by TAP-tagging Tif6- The protein content of 60 S preribosomes associated with Tif6p in a Deltaefl1 strain are obtained at a lower yield but have, surprisingly, a protein composition that is a priori similar to that of wild-type and the suppressor strain- [PUBMED: 16095611] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.