Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us J. Biol. Chem. Jul (2005); 280(26):24532-8 Yeast mRNA Poly-A- tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly-A- nuclease activity- Dheur S, Nykamp KR, Viphakone N, Swanson MS, Minvielle-Sebastia L CNRS UMR 5095, Institut de Biochimie et Genetique Cellulaires, 1 rue Camille Saint-Saens, F-33077 Bordeaux cedex, France- Abstract: Regulation of poly-A- tail length during mRNA 3'-end formation requires a specific poly-A--binding protein in addition to the cleavage-polyadenylation machinery- The mechanism that controls polyadenylation in mammals is well understood and involves the nuclear poly-A--binding protein PABPN1- In contrast, poly-A- tail length regulation is poorly understood in yeast- Previous studies have suggested that the major cytoplasmic poly-A--binding protein Pab1p acts as a length control factor in conjunction with the Pab1p-dependent poly-A- nuclease PAN, to regulate poly-A- tail length in an mRNA specific manner- In contrast, we recently showed that Nab2p regulates polyadenylation during de novo synthesis, and its nuclear location is more consistent with a role in 3'-end processing than that of cytoplasmic Pab1p- Here, we investigate whether PAN activity is required for de novo poly-A- tail synthesis- Components required for mRNA 3'-end formation were purified from wild-type and pan mutant cells- In both situations, 3'-end formation could be reconstituted whether Nab2p or Pab1p was used as the poly-A- tail length control factor- However, polyadenylation was more efficient and physiologically more relevant in the presence of Nab2p as opposed to Pab1p- Moreover, cell immunofluorescence studies confirmed that PAN subunits are localized in the cytoplasm which suggests that cytoplasmic Pab1p and PAN may act at a later stage in mRNA metabolism- Based on these findings, we propose that Nab2p is necessary and sufficient to regulate poly-A- tail length during de novo synthesis in yeast- [PUBMED: 15894541] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.