The BioGRID | PUBLICATION VIEW

	J. Cell Biol. Jan (2003); 160(2):201-12
The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation.
Diao A, Rahman D, Pappin DJ, Lucocq J, Lowe M
School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK.
Abstract: Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. We have used a biochemical approach to characterize these proteins and report here the identification of golgin-84 as a novel mitotic target. Using cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is enriched on tubules emanating from the lateral edges of, and often connecting, Golgi stacks. Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon. Strikingly, the Golgi ribbon is converted into mini-stacks constituting only approximately 25% of the volume of a normal Golgi apparatus upon golgin-84 depletion. These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. Our results suggest that golgin-84 plays a key role in the assembly and maintenance of the Golgi ribbon in mammalian cells.
[PUBMED: 12538640]