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	J. Biol. Chem. May (2005); 280(18):18442-51
Reconstitution of Nup157 and Nup145N into the Nup84 complex-
Lutzmann M, Kunze R, Stangl K, Stelter P, Toth KF, Bottcher B, Hurt E
Biochemie-Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany-
Abstract: About 30 different nucleoporins -Nups- constitute the nuclear pore complex- We have affinity-purified 28 of these nuclear pore proteins and identified new nucleoporin interactions by this analysis- We found that Nup157 and Nup170, two members of the large structural Nups, and the Gly-Leu-Phe-Gly nucleoporin Nup145N specifically co-purified with members of the Nup84 complex- In addition, Nup145N co-enriched during Nup157 purification- By in vitro reconstitution, we demonstrate that Nup157 and Nup145N form a nucleoporin subcomplex- Moreover, we show that Nup157 and Nup145N bind to the heptameric Nup84 complex- This assembly thus represents approximately one-third of all nucleoporins- To characterize Nup157 structurally, we purified and analyzed it by electron microscopy- Nup157 is a hollow sphere that resembles a clamp or a gripping hand- Thus, we could reconstitute an interaction between a large structural Nup, an FG repeat Nup, and a major structural module of the nuclear pore complex-
[PUBMED: 15741174]