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| Genetics Sep (2006); 174(1):215-27 | |
| The conserved ATPase Get3-Arr4 modulates the activity of membrane-associated proteins in Saccharomyces cerevisiae- | |
| Auld KL, Hitchcock AL, Doherty HK, Frietze S, Huang LS, Silver PA | |
| Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA- | |
| Abstract: The regulation of cellular membrane dynamics is crucial for maintaining proper cell growth and division- The Cdc48-Npl4-Ufd1 complex is required for several regulated membrane-associated processes as part of the ubiquitin-proteasome system, including ER-associated degradation and the control of lipid composition in yeast- In this study we report the results of a genetic screen in Saccharomyces cerevisiae for extragenic suppressors of a temperature-sensitive npl4 allele and the subsequent analysis of one suppressor, GET3-ARR4- The GET3 gene encodes an ATPase with homology to the regulatory component of the bacterial arsenic pump- Mutants of GET3 rescue several phenotypes of the npl4 mutant and transcription of GET3 is coregulated with the proteasome, illustrating a functional relationship between GET3 and NPL4 in the ubiquitin-proteasome system- We have further found that Get3 biochemically interacts with the trans-membrane domain proteins Get1-Mdm39 and Get2-Rmd7 and that Deltaget3 is able to suppress phenotypes of get1 and get2 mutants, including sporulation defects- In combination, our characterization of GET3 genetic and biochemical interactions with NPL4, GET1, and GET2 implicates Get3 in multiple membrane-dependent pathways- | |
| [PUBMED: 16816426] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |