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| J. Cell. Sci. Jun (2006); 119:2604-12 | |
| The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus- | |
| Yahara N, Sato K, Nakano A | |
| Molecular Membrane Biology Laboratory, RIKEN Discovery Research Institute, Hirosawa, Wako, Saitama 351-0198, Japan- | |
| Abstract: ADP-ribosylation factors -Arfs-, key regulators of intracellular membrane traffic, are known to exert multiple roles in vesicular transport- We previously isolated eight temperature-sensitive -ts- mutants of the yeast ARF1 gene, which showed allele-specific defects in protein transport, and classified them into three groups of intragenic complementation- In this study, we show that the overexpression of Glo3p, one of the GTPase-activating proteins of Arf1p -ArfGAP-, suppresses the ts growth of a particular group of the arf1 mutants -arf1-16 and arf1-17-- Other ArfGAPs do not show such a suppression activity- All these ArfGAPs show sequence similarity in the ArfGAP catalytic domain, but are divergent in the rest of molecules- By domain swapping analysis of Glo3p and another ArfGAP, Gcs1p, we have shown that the non-catalytic C-terminal region of Glo3p is required for the suppression of the growth defect in the arf1 ts mutants- Interestingly, Glo3p and its homologues from other eukaryotes harbor a well-conserved repeated ISSxxxFG sequence near the C-terminus, which is not found in Gcs1p and its homologues- We name this region the Glo3 motif and present evidence that the motif is required for the function of Glo3p in vivo- | |
| [PUBMED: 16735437] |   |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |