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	J. Cell Biol. Aug (2006); 174(5):653-63
Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation-
Montpetit B, Hazbun TR, Fields S, Hieter P
Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada-
Abstract: Posttranslational modification by the ubiquitin-like protein SUMO -small ubiquitin-like modifier- is emerging as an important regulator in many cellular processes, including genome integrity- In this study, we show that the kinetochore proteins Ndc10, Bir1, Ndc80, and Cep3, which mediate the attachment of chromosomes to spindle microtubules, are sumoylated substrates in budding yeast- Furthermore, we show that Ndc10, Bir1, and Cep3 but not Ndc80 are desumoylated upon exposure to nocodazole, highlighting the possibility of distinct roles for sumoylation in modulating kinetochore protein function and of a potential link between the sumoylation of kinetochore proteins and mitotic checkpoint function- We find that lysine to arginine mutations that eliminate the sumoylation of Ndc10 cause chromosome instability, mislocalization of Ndc10 from the mitotic spindle, abnormal anaphase spindles, and a loss of Bir1 sumoylation- These data suggest that sumoylation of Ndc10 and other kinetochore proteins play a critical role during the mitotic process-
[PUBMED: 16923829]