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	Genetics Jun (2006); 173(2):557-67
The requirements for COMPASS and Paf1 in transcriptional silencing and methylation of histone H3 in Saccharomyces cerevisiae-
Mueller JE, Canze M, Bryk M
Department of Biochemistry and Biophysics, Texas A-M University, College Station, Texas 77843-2128, USA-
Abstract: The Set1-containing complex, COMPASS, methylates histone H3 on lysine 4 -K4- in Saccharomyces cerevisiae- Despite the preferential association of K4-trimethylated H3 with regions of the genome that are transcribed by RNA polymerase II, transcriptional silencing is one of the few cases in S- cerevisiae where histone-methylation defects have a clear effect on gene expression- To better understand the role of COMPASS in transcriptional silencing, we have determined which members of COMPASS are required for silencing at the ribosomal DNA locus -rDNA-, a telomere, and the silent mating loci -HM- using Northern analyses- Our findings indicate that most members of COMPASS are required for silencing at the rDNA and telomere, while none are required for silencing of endogenous genes at the HM loci- To complement gene-expression analysis, quantitative Western blot experiments were performed to determine the members of COMPASS that are required for methylation of histone H3- While most are required for trimethylation, cells lacking certain COMPASS proteins maintain reduced levels of K4 mono- and dimethylated H3, suggesting that some COMPASS members have redundant function- Finally, we show Paf1 is required for silencing and K4-methylated H3 at the rDNA, suggesting a possible direct role for K4-methylated H3 in gene silencing-
[PUBMED: 16582434]

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