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| Mol. Syst. Biol. (2005); 1:2005.0026 | |
| Gene function prediction from congruent synthetic lethal interactions in yeast- | |
| Ye P, Peyser BD, Pan X, Boeke JD, Spencer FA, Bader JS | |
| Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD, USA- | |
| Abstract: We predicted gene function using synthetic lethal genetic interactions between null alleles in Saccharomyces cerevisiae- Phenotypic and protein interaction data indicate that synthetic lethal gene pairs function in parallel or compensating pathways- Congruent gene pairs, defined as sharing synthetic lethal partners, are in single pathway branches- We predicted benomyl sensitivity and nuclear migration defects using congruence; these phenotypes were uncorrelated with direct synthetic lethality- We also predicted YLL049W as a new member of the dynein-dynactin pathway and provided new supporting experimental evidence- We performed synthetic lethal screens of the parallel mitotic exit network -MEN- and Cdc14 early anaphase release pathways required for late cell cycle- Synthetic lethal interactions bridged genes in these pathways, and high congruence linked genes within each pathway- Synthetic lethal interactions between MEN and all components of the Sin3-Rpd3 histone deacetylase revealed a novel function for Sin3-Rpd3 in promoting mitotic exit in parallel to MEN- These in silico methods can predict phenotypes and gene functions and are applicable to genomic synthetic lethality screens in yeast and analogous RNA interference screens in metazoans- | |
| [PUBMED: 16729061] |   |
| Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. |
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| BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9. |