Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us Genetics Feb (2006); 172(2):795-809 The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles- Wilson B, Erdjument-Bromage H, Tempst P, Cairns BR Howard Hughes Medical Institute, University of Utah School of Medicine, Salt Lake City 84112, USA- Abstract: RSC is an essential and abundant ATP-dependent chromatin remodeling complex from Saccharomyces cerevisiae- Here we show that the RSC components Rsc7-Npl6 and Rsc14-Ldb7 interact physically and-or functionally with Rsc3, Rsc30, and Htl1 to form a module important for a broad range of RSC functions- A strain lacking Rsc7 fails to properly assemble RSC, which confers sensitivity to temperature and to agents that cause DNA damage, microtubule depolymerization, or cell wall stress -likely via transcriptional misregulation-- Cells lacking Rsc14 display sensitivity to cell wall stress and are deficient in the assembly of Rsc3 and Rsc30- Interestingly, certain rsc7delta and rsc14delta phenotypes are suppressed by an increased dosage of Rsc3, an essential RSC member with roles in cell wall integrity and spindle checkpoint pathways- Thus, Rsc7 and Rsc14 have different roles in the module as well as sharing physical and functional connections to Rsc3- Using a genetic array of nonessential null mutations -SGA- we identified mutations that are sick-lethal in combination with the rsc7delta mutation, which revealed connections to a surprisingly large number of chromatin remodeling complexes and cellular processes- Taken together, we define a protein module on the RSC complex with links to a broad spectrum of cellular functions- [PUBMED: 16204215] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.