The BioGRID | PUBLICATION VIEW

	Mol. Cell. Neurosci. Dec (2003); 24(4):1038-50
Rb binding protein Che-1 interacts with Tau in cerebellar granule neurons- Modulation during neuronal apoptosis-
Barbato C, Corbi N, Canu N, Fanciulli M, Serafino A, Ciotti M, Libri V, Bruno T, Amadoro G, De Angelis R, Calissano P, Passananti C
Istituto di Neurobiologia e Medicina Molecolare, CNR, Viale Marx 43, 00137 Rome, Italy-
Abstract: Che-1 is a recently identified human Rb binding protein that inhibits the Rb growth-suppressing function and regulates cell proliferation- Che-1 contacts the Rb and competes with HDAC1 for Rb-binding site, removing HDAC1 from the Rb-E2F cell cycle-regulated promoters- We have investigated the expression of Che-1 in neuronal cells and we showed that Che-1 directly interacts with Tau- Tau is a microtubule-associated protein involved in the assembly and stabilization of neuronal microtubules network that plays a crucial role modulating neuronal morphogenesis, axonal shape, and transport- In rat cerebellar granule neurons -CGNs- Che-1 partially colocalizes with Tau in the cytoplasm- Che-1 binds the amino-terminal region of Tau protein, which is not involved in microtubule interactions- Tau and Che-1 endogenous proteins coimmunoprecipitate from CGNs cellular lysates- In addition, Che-1-Tau interaction was demonstrated both in overexpressing COS-7 cells and CGNs by FRET analysis- Finally, we observed that Tau-Che-1 interaction is modulated during neuronal apoptosis-
[PUBMED: 14697667]