Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us Genes Dev. May (2006); 20(10):1294-307 Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes- Fleischer TC, Weaver CM, McAfee KJ, Jennings JL, Link AJ Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA- Abstract: Translation regulation is a critical means by which cells control growth, division, and apoptosis- To gain further insight into translation and related processes, we performed multifaceted mass spectrometry-based proteomic screens of yeast ribosomal complexes and discovered an association of 77 uncharacterized yeast proteins with ribosomes- Immunoblotting revealed an EDTA-dependent cosedimentation with ribosomes in sucrose gradients for 11 candidate translation-machinery-associated -TMA- proteins- Tandem affinity purification linked one candidate, LSM12, to the RNA processing proteins PBP1 and PBP4- A second candidate, TMA46, interacted with RBG1, a GTPase that interacts with ribosomes- By adapting translation assays to high-throughput screening methods, we showed that null yeast strains harboring deletions for several of the TMA genes had alterations in protein synthesis rates -TMA7 and TMA19-, susceptibility to drugs that inhibit translation -TMA7-, translation fidelity -TMA20-, and polyribosome profiles -TMA7, TMA19, and TMA20-- TMA20 has significant sequence homology with the oncogene MCT-1- Expression of human MCT-1 in the Deltatma20 yeast mutant complemented translation-related defects, strongly implying that MCT-1 functions in translation-related processes- Together these findings implicate the TMA proteins and, potentially, their human homologs, in translation related processes- [PUBMED: 16702403] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.