The BioGRID | PUBLICATION VIEW

	EMBO J. Mar (2006); 25(5):943-54
Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi-
Trautwein M, Schindler C, Gauss R, Dengjel J, Hartmann E, Spang A
Friedrich Miescher Laboratorium, Max Planck Gesellschaft, Tubingen, Germany-
Abstract: In Saccharomyces cerevisiae, the synthesis of chitin is temporally and spatially regulated through the transport of Chs3p -chitin synthase III- to the plasma membrane in the bud neck region- Traffic of Chs3p from the trans-Golgi network -TGN--early endosome to the plasma membrane requires the function of Chs5p and Chs6p- Chs6p belongs to a family of four proteins that we have named ChAPs for Chs5p-Arf1p-binding Proteins- Here, we show that all ChAPs physically interact not only with Chs5p but also with the small GTPase Arf1p- A short sequence at the C-terminus of the ChAPs is required for protein function and the ability to bind to Chs5p- Simultaneous disruption of two members, Deltabud7 and Deltabch1, phenocopies a Deltachs6 or Deltachs5 deletion with respect to Chs3p transport- Moreover, the ChAPs interact with each other and can form complexes- In addition, they are all at least partially localized to the TGN in a Chs5p-dependent manner- Most importantly, several ChAPs can interact physically with Chs3p- We propose that the ChAPs facilitate export of cargo out of the Golgi-
[PUBMED: 16498409]