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	Mol. Biol. Cell Apr (2006); 17(4):1959-70
The Saccharomyces cerevisiae spindle pole body -SPB- component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p-
Araki Y, Lau CK, Maekawa H, Jaspersen SL, Giddings TH, Schiebel E, Winey M
The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 4BX, United Kingdom-
Abstract: The spindle pole body -SPB- in Saccharomyces cerevisiae functions to nucleate and organize spindle microtubules, and it is embedded in the nuclear envelope throughout the yeast life cycle- However, the mechanism of membrane insertion of the SPB has not been elucidated- Ndc1p is an integral membrane protein that localizes to SPBs, and it is required for insertion of the SPB into the nuclear envelope during SPB duplication- To better understand the function of Ndc1p, we performed a dosage suppressor screen using the ndc1-39 temperature-sensitive allele- We identified an essential SPB component, Nbp1p- NBP1 shows genetic interactions with several SPB genes in addition to NDC1, and two-hybrid analysis revealed that Nbp1p binds to Ndc1p- Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB- Immunoelectron microscopy confirmed that Nbp1p localizes to the SPB, suggesting a function at this location- Consistent with this hypothesis, nbp1-td -a degron allele- cells fail in SPB duplication upon depletion of Nbp1p- Importantly, these cells exhibit a "dead" SPB phenotype, similar to cells mutant in MPS2, NDC1, or BBP1- These results demonstrate that Nbp1p is a SPB component that acts in SPB duplication at the point of SPB insertion into the nuclear envelope-
[PUBMED: 16436507]

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