The BioGRID | PUBLICATION VIEW

	J. Cell Biol. Dec (2005); 171(6):955-65
The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control-
Dilworth DJ, Tackett AJ, Rogers RS, Yi EC, Christmas RH, Smith JJ, Siegel AF, Chait BT, Wozniak RW, Aitchison JD
Institute for Systems Biology, Seattle, WA 98103, USA-
Abstract: Nuclear pore complexes -NPCs- govern macromolecular transport between the nucleus and cytoplasm and serve as key positional markers within the nucleus- Several protein components of yeast NPCs have been implicated in the epigenetic control of gene expression- Among these, Nup2p is unique as it transiently associates with NPCs and, when artificially tethered to DNA, can prevent the spread of transcriptional activation or repression between flanking genes, a function termed boundary activity- To understand this function of Nup2p, we investigated the interactions of Nup2p with other proteins and with DNA using immunopurifications coupled with mass spectrometry and microarray analyses- These data combined with functional assays of boundary activity and epigenetic variegation suggest that Nup2p and the Ran guanylyl-nucleotide exchange factor, Prp20p, interact at specific chromatin regions and enable the NPC to play an active role in chromatin organization by facilitating the transition of chromatin between activity states-
[PUBMED: 16365162]