Search Organism: All OrganismsArabidopsis thalianaBacillus subtilis 168Bos taurusCaenorhabditis elegansCanis familiarisDanio rerioDrosophila melanogasterEquus caballusEscherichia coli K12Gallus gallusHomo sapiensMacaca mulattaMus musculusNematostella vectensisOryza sativaPan troglodytesRattus norvegicusSaccharomyces cerevisiaeSchizosaccharomyces pombeStrongylocentrotus purpuratusTakifugu rubripesXenopus laevis home help / support contribute downloads mirrors about us Mol. Biol. Cell Feb (2006); 17(2):1006-17 Depletion of phosphatidylcholine in yeast induces shortening and increased saturation of the lipid acyl chains- evidence for regulation of intrinsic membrane curvature in a eukaryote- Boumann HA, Gubbens J, Koorengevel MC, Oh CS, Martin CE, Heck AJ, Patton-Vogt J, Henry SA, de Kruijff B, de Kroon AI Department of Biochemistry of Membranes, Bijvoet Institute, Utrecht University, 3584 CH Utrecht, The Netherlands- Abstract: To study the consequences of depleting the major membrane phospholipid phosphatidylcholine -PC-, exponentially growing cells of a yeast cho2opi3 double deletion mutant were transferred from medium containing choline to choline-free medium- Cell growth did not cease until the PC level had dropped below 2% of total phospholipids after four to five generations- Increasing contents of phosphatidylethanolamine -PE- and phosphatidylinositol made up for the loss of PC- During PC depletion, the remaining PC was subject to acyl chain remodeling with monounsaturated species replacing diunsaturated species, as shown by mass spectrometry- The remodeling of PC did not require turnover by the SPO14-encoded phospholipase D- The changes in the PC species profile were found to reflect an overall shift in the cellular acyl chain composition that exhibited a 40% increase in the ratio of C16 over C18 acyl chains, and a 10% increase in the degree of saturation- The shift was stronger in the phospholipid than in the neutral lipid fraction and strongest in the species profile of PE- The shortening and increased saturation of the PE acyl chains were shown to decrease the nonbilayer propensity of PE- The results point to a regulatory mechanism in yeast that maintains intrinsic membrane curvature in an optimal range- [PUBMED: 16339082] Copyright © 2009, Tyers Lab, The Samuel Lunenfeld Research Institute, Mount Sinai Hospital, All Rights Reserved. terms and conditions - privacy policy - Osprey Network Visualization System BioGRID: A General Repository for Interaction Datasets. Chris Stark, Bobby-Joe Breitkreutz, Teresa Reguly, Lorrie Boucher, Ashton Breitkreutz, Mike Tyers. Nucleic Acids Res. Jan 1;34:D535-9.