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	FEBS Lett. Sep (1995); 372(1):59-64
A novel tau-tubulin kinase from bovine brain.
Takahashi M, Tomizawa K, Sato K, Ohtake A, Omori A
Mitsubishi Kasei Institute of Life Sciences (Project 2), Tokyo, Japan.
Abstract: During purification of tau protein kinase I and II from the bovine brain extract, a new tau protein kinase was detected and purified with phosphocellulose, gel filtration, S-Sepharose and AF-Heparin column chromatography. The molecular mass of the enzyme was determined to be 32 kDa by gel filtration and activity staining on SDS-PAGE. The enzyme is a Ser/Thr protein kinase phosphorylating tau, beta-tubulin, MAP2 and alpha-casein. Employing many synthetic peptides, the recognition site of this enzyme appears to be -SR-. The enzyme requires no second messenger and is inhibited with high concentration of heparin, but not by inhibitors of CKI. These results indicate that this enzyme, tau-tubulin kinase is novel and distinct from TPKI, II and CKI, II.
[PUBMED: 7556643]