The BioGRID | RSP5 - YER125W : Saccharomyces cerevisiae search results

Name

Aliases

Description

Evidence Code(s)

Role

Source(s)

RPO21

RPB1

RPB220

SUA8

YDL140C

RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime|Rpo21p; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

hit	Ho Y (2002)

Affinity Capture-Western

hit	Morris DP (2000)
hit	Chang A (2000)
hit	Somesh BP (2007)

Far Western

hit	Morris DP (2000)
bait	Chang A (2000)

Reconstituted Complex

hit	Wang G (1999)

Two-hybrid

bait/hit	Wu X (2001)
bait	Chang A (2000)

Biochemical Activity

hit	Huibregtse JM (1997)
hit	Somesh BP (2005)

Dosage Rescue

hit	Wu X (2001)

BUL1

DAG1

RDS1

SMM2

YMR275C

Bul1p|Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

hit	Ho Y (2002)
hit	Kee Y (2005)
bait	Krogan NJ (2006)

Affinity Capture-Western

bait/hit	Yashiroda H (1996)
bait/hit	Yashiroda H (1998)

Two-hybrid

bait/hit	Yashiroda H (1996)
bait/hit	Yashiroda H (1998)

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)
hit	Stawiecka-Mirota M (2007)

Dosage Lethality

bait	Yashiroda H (1996)
hit	Kus B (2005)

SNA3

YJL151C

Integral membrane protein localized to vacuolar intralumenal vesicles, computational analysis of large-scale protein-protein interaction data suggests a possible role in either cell wall synthesis or protein-vacuolar targeting|Sna3p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Gupta R (2007)

Affinity Capture-Western

hit	Oestreich AJ (2007)
hit	Watson H (2007)
bait/hit	Stawiecka-Mirota M (2007)

Reconstituted Complex

bait	McNatt MW (2007)
hit	Watson H (2007)
hit	Stawiecka-Mirota M (2007)

UBP2

YOR124C

Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins, cleaves at the C terminus of ubiquitin fusions; capable of cleaving polyubiquitin and possesses isopeptidase activity|Ubp2p; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

hit	Kee Y (2005)
bait	Krogan NJ (2006)
bait	Gavin AC (2006)
hit	Collins SR (2007)

Affinity Capture-Western

hit	Kee Y (2005)

Dosage Growth Defect

hit	Kee Y (2005)

Phenotypic Suppression

hit	Kee Y (2005)

MGA2

YIR033W

ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting|Mga2p; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

bait	Shcherbik N (2003)

Reconstituted Complex

bait	Shcherbik N (2003)
bait	Shcherbik N (2004)
hit	Bhattacharya S (2008)

Biochemical Activity

hit	Bhattacharya S (2008)

Affinity Capture-Western

bait	Bhattacharya S (2008)

SPT23

YKL020C

ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Mga2p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting|Spt23p; protein coding

Gene Ontology: [View List]

Reconstituted Complex

hit	Shcherbik N (2004)
hit	Bhattacharya S (2008)

Affinity Capture-Western

bait	Shcherbik N (2004)
bait	Bhattacharya S (2008)

Biochemical Activity

hit	Kee Y (2005)
hit	Bhattacharya S (2008)

RUP1

YOR138C

Protein involved in regulation of Rsp5p, which is an essential HECT ubiquitin ligase; required for binding of Rsp5p to Ubp2p; contains an UBA domain|Rup1p; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

hit	Kee Y (2005)

Reconstituted Complex

hit	Kee Y (2005)

Affinity Capture-MS

bait	Krogan NJ (2006)
hit	Collins SR (2007)

Dosage Growth Defect

hit	Kee Y (2005)

Phenotypic Suppression

hit	Kee Y (2005)

RVS167

YDR388W

Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin|Rvs167p; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

bait	Ho Y (2002)

Reconstituted Complex

bait/hit

Stamenova SD (2004)

Two-hybrid

bait	Stamenova SD (2004)

Affinity Capture-Western

hit	Stamenova SD (2004)

Biochemical Activity

hit	Gupta R (2007)

RCR1

YBR005W

Protein of the ER membrane involved in cell wall chitin deposition; may function in the endosomal-vacuolar trafficking pathway, helping determine whether plasma membrane proteins are degraded or routed to the plasma membrane|Rcr1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

Affinity Capture-Western

hit	Gupta R (2007)
bait	Imai K (2007)

Two-hybrid

bait	Imai K (2007)

YJL084C

ALY2

Aly2p|Cytoplasmic protein of unknown function that interacts with the cyclin Pcl7p; phosphorylated in vitro by the cyclin-CDK complex, Pcl7p-Pho85p; identified as a potential Cdc28p substrate; mRNA is cell cycle regulated, peaking in M phase; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

bait	Krogan NJ (2006)
hit	Collins SR (2007)

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

Affinity Capture-Western

hit	Gupta R (2007)

DIA1

YMR316W

Dia1p|Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; protein coding

Gene Ontology: [View List]

Two-hybrid

bait	Ito T (2001)

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

Affinity Capture-Western

hit	Gupta R (2007)

YDL203C

ACK1

Ack1p|Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

hit	Kus B (2005)
hit	Gupta R (2007)

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

ROD1

YOR018W

Membrane protein; overexpression confers resistance to the GST substrate o-dinitrobenzene as well as to zinc and calcium; contains 2 PY motifs, which are required for Rod1p interaction with Rsp5p, a hect-type ubiquitin ligase|Rod1p; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

bait	Andoh T (2002)

Reconstituted Complex

bait	Andoh T (2002)
hit	Gupta R (2007)

Biochemical Activity

hit	Gupta R (2007)

YLR392C

Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR392C is not an essential gene|Ylr392cp; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

hit	Ho Y (2002)

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

Affinity Capture-Western

hit	Gupta R (2007)

YGR068C

Putative protein of unknown function; YGR068C is not an essential gene.|Ygr068cp; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

Affinity Capture-Western

hit	Gupta R (2007)

YHR131C

Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm|Yhr131cp; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

bait	Krogan NJ (2006)
hit	Collins SR (2007)

Biochemical Activity

hit	Gupta R (2007)

ROG3

YFR022W

Protein that binds to Rsp5p, which is a hect-type ubiquitin ligase, via its 2 PY motifs; has similarity to Rod1p; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant|Rog3p; protein coding

Gene Ontology: [View List]

Reconstituted Complex

hit	Andoh T (2002)

Affinity Capture-Western

bait	Andoh T (2002)

Biochemical Activity

hit	Gupta R (2007)

YKR021W

ALY1

Aly1p|Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein coding

Gene Ontology: [View List]

Affinity Capture-MS

hit	Collins SR (2007)

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

HSE1

YHL002W

Hse1p|Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes; protein coding

Gene Ontology: [View List]

Two-hybrid

bait	Bowers K (2004)

Affinity Capture-Western

bait	Ren J (2007)

Reconstituted Complex

hit	Ren J (2007)

SNA4

YDL123W

Protein of unknown function, localized to the vacuolar outer membrane|Sna4p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Gupta R (2007)

Reconstituted Complex

hit	Gupta R (2007)

Affinity Capture-Western

hit	Gupta R (2007)

ROG1

YGL144C

Protein with putative serine active lipase domain|Rog1p; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

bait/hit

Andoh T (2000)

Reconstituted Complex

bait	Andoh T (2000)

Synthetic Rescue

hit	Andoh T (2000)

RIM4

YHL024W

Putative RNA-binding protein required for the expression of early and middle sporulation genes|Rim4p; protein coding