The BioGRID Database Seperator
Search
Organism:

BioGRID | Network Layout for VAC8 for Saccharomyces cerevisiae
[JPG] [PNG] [SVG]		 The BioGRID Database Seperator

VAC8

 Saccharomyces cerevisiae
The BioGRID Database Seperator
Aliases: YEB3, YEL013W
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Description: Phosphorylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions|Vac8p; protein coding
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Links: [Entrez-Gene]  [GeneDB]  [Germ Online]  [SGD]  
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Gene Ontology: 8 total GO annotations for this record. [view list]
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Download interactions associated with VAC8

VAC8 was identified with 45 protein interactions and 21 genetic interactions
Displaying:
 Name Aliases The BioGRID Database Seperator Description
Evidence Code(s)
Role Source(s)
The BioGRID Database Seperator
 ATG13
  • APG13
  • YPR185W
    		•  The BioGRID Database Seperator Atg13p|Phosphorylated protein that interacts with Vac8p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway and autophagy; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    hit Scott SV (2000)
    bait Cheong H (2005)
    hit Tang F (2006)
    Affinity Capture-Western
    hit Scott SV (2000)
    bait Pan X (2000)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VAB2
  • VAB31
  • YEL005C
    		•  The BioGRID Database Seperator Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern|Vab2p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Pan X (2000)
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     PFA3
  • YNL326C
    		•  The BioGRID Database Seperator Palmitoyltransferase for Vac8p, required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions|Pfa3p; protein coding
    Gene Ontology: [View List]
    Biochemical Activity
    bait Smotrys JE (2005)
    Co-localization
    hit Hou H (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     MEH1
  • EGO1
  • GSE2
  • YKR007W
    		•  The BioGRID Database Seperator Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification|Meh1p; protein coding
    Gene Ontology: [View List]
    Co-fractionation
    hit Hou H (2005)
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     NVJ1
  • VAB36
  • YHR195W
    		•  The BioGRID Database Seperator Nuclear envelope protein that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)|Nvj1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Pan X (2000)
    Two-hybrid
    hit Pan X (2000)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VAM3
  • PTH1
  • YOR106W
    		•  The BioGRID Database Seperator Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein trafficking; member of the syntaxin family of proteins|Vam3p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Veit M (2001)
    Reconstituted Complex
    hit Veit M (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VAC17
  • YCL062W
  • YCL063W
    		•  The BioGRID Database Seperator Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p|Vac17p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Tang F (2003)
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YKT6
  • YKL196C
    		•  The BioGRID Database Seperator Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus|Ykt6p; protein coding
    Gene Ontology: [View List]
    Reconstituted Complex
    bait/hit Dietrich LE (2004)
    bait Dietrich LE (2004)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CDC73
  • YLR418C
    		•  The BioGRID Database Seperator Cdc73p|Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p, distinct from Srb-containing Pol II complexes; required for expression of certain genes, modification of some histones, and telomere maintenance; protein coding
    Gene Ontology: [View List]
    Synthetic Lethality
    bait Tong AH (2004)
    bait Krogan NJ (2003)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     TOR1
  • DRR1
  • YJR066W
    		•  The BioGRID Database Seperator PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis|Tor1p; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement
    bait/hit Schuldiner M (2005)
    Synthetic Growth Defect
    bait Zurita-Martinez SA (2007)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     COT1
  • YOR316C
    		•  The BioGRID Database Seperator Cot1p|Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     GCD7
  • YLR291C
    		•  The BioGRID Database Seperator Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression|Gcd7p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Ho Y (2002)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     FTH1
  • YBR207W
    		•  The BioGRID Database Seperator Fth1p|Putative high affinity iron transporter involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VMA7
  • YGR020C
    		•  The BioGRID Database Seperator Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane|Vma7p; protein coding
    Gene Ontology: [View List]
    PCA
    hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SWH1
  • OSH1
  • YAR044W
  • YAR042W
    		•  The BioGRID Database Seperator Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction|Swh1p; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SNA4
  • YDL123W
    		•  The BioGRID Database Seperator Protein of unknown function, localized to the vacuolar outer membrane|Sna4p; protein coding
    Gene Ontology: [View List]
    PCA
    bait Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     GTR1
  • YML121W
    		•  The BioGRID Database Seperator Cytoplasmic GTP binding protein and negative regulator, with homolog Gtr2p, of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport; has homology to human RagA and RagB|Gtr1p; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     IML1
  • YJR138W
    		•  The BioGRID Database Seperator Iml1p|Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; protein coding
    Gene Ontology: [View List]
    PCA
    hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YFR035C
  • YFR035C
    		•  The BioGRID Database Seperator Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein|Yfr035cp; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     HXT1
  • HOR4
  • YHR094C
    		•  The BioGRID Database Seperator Hxt1p|Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SNA3
  • YJL151C
    		•  The BioGRID Database Seperator Integral membrane protein localized to vacuolar intralumenal vesicles, computational analysis of large-scale protein-protein interaction data suggests a possible role in either cell wall synthesis or protein-vacuolar targeting|Sna3p; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     PMC1
  • YGL006W
    		•  The BioGRID Database Seperator Pmc1p|Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a; protein coding
    Gene Ontology: [View List]
    PCA
    hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DPP1
  • ZRG1
  • YDR284C
    		•  The BioGRID Database Seperator Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism|Dpp1p; protein coding
    Gene Ontology: [View List]
    PCA
    bait/hit Tarassov K (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ACT1
  • ABY1
  • END7
  • YFL039C
    		•  The BioGRID Database Seperator Act1p|Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    hit Wang YX (1998)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     BUD32
  • LDB14
  • YGR262C
    		•  The BioGRID Database Seperator Bud32p|Protein kinase proposed to be involved in bud-site selection, telomere uncapping and elongation, and transcription; component of the EKC/KEOPS protein complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    bait Lopreiato R (2004)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     JSN1
  • PUF1
  • YJR091C
    		•  The BioGRID Database Seperator Jsn1p|Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    bait Ito T (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YEL043W
  • YEL043W
    		•  The BioGRID Database Seperator Predicted cytoskeleton protein involved in intracellular signalling based on quantitative analysis of protein-protein interaction maps; may interact with ribosomes, based on co-purification studies; contains fibronectin type III domain fold|Yel043wp; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     RFC2
  • YJR068W
    		•  The BioGRID Database Seperator Rfc2p|Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Ho Y (2002)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     FMP41
  • YNL168C
    		•  The BioGRID Database Seperator Fmp41p|Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VTI1
  • YMR197C
    		•  The BioGRID Database Seperator Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways|Vti1p; protein coding
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Veit M (2001)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VID21
  • EAF1
  • YDR359C
    		•  The BioGRID Database Seperator Component of the NuA4 histone acetyltransferase complex|Eaf1p; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     TAO3
  • PAG1
  • YIL129C
    		•  The BioGRID Database Seperator Protein involved in cell morphogenesis and proliferation, associated with protein kinase Cbk1p; mutants activate OCH1 transcription|Tao3p; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     BEM1
  • SRO1
  • YBR200W
    		•  The BioGRID Database Seperator Bem1p|Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p; protein coding
    Gene Ontology: [View List]
    Co-fractionation
    bait Xu H (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     TCO89
  • YPL180W
    		•  The BioGRID Database Seperator Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin|Tco89p; protein coding
    Gene Ontology: [View List]
    Two-hybrid
    hit Tang F (2006)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YCK3
  • CKI3
  • YER123W
    		•  The BioGRID Database Seperator Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of the HOPS complex subunit, Vps41p; shares overlapping essential functions with Hrr25p|Yck3p; protein coding
    Gene Ontology: [View List]
    Co-fractionation
    hit Hou H (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     NCE102
  • NCE2
  • YPR149W
    		•  The BioGRID Database Seperator Nce102p|Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); protein coding
    Gene Ontology: [View List]
    Affinity Capture-MS
    hit Collins SR (2007)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC27
  • YGL137W
    		•  The BioGRID Database Seperator Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP|Sec27p; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement
    bait Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     STE24
  • AFC1
  • PIO2
  • YJR117W
    		•  The BioGRID Database Seperator Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans|Ste24p; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement
    bait Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ICE2
  • YIL090W
    		•  The BioGRID Database Seperator Ice2p|Integral ER membrane protein with type-III transmembrane domains; mutations cause defects in cortical ER morphology in both the mother and daughter cells; protein coding
    Gene Ontology: [View List]
    Phenotypic Suppression
    bait/hit Schuldiner M (2005)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     EAF7
  • YNL136W
    		•  The BioGRID Database Seperator Eaf7p|Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A; protein coding
    Gene Ontology: [View List]
    Synthetic Growth Defect
    bait Lin YY (2008)
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SCS7
  • FAH1
  • YMR272C
    		•  The BioGRID Database Seperator Scs7p|Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth; protein coding
    Gene Ontology: [View List]
    Phenotypic Enhancement