BioGRID | Network Layout for IRE1 for Saccharomyces cerevisiae

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IRE1

Saccharomyces cerevisiae

Aliases: ERN1, YHR079C

Description: Ire1p|Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress; protein coding

Links: [Entrez-Gene] [GeneDB] [Germ Online] [SGD]

Gene Ontology: 11 total GO annotations for this record. [view list]

Download interactions associated with IRE1

Name

Aliases

Description

Evidence Code(s)

Role

Source(s)

GRP78

YJL034W

ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p|Kar2p; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

bait/hit	Okamura K (2000)
hit	Todd-Corlett A (2007)
hit	Kimata Y (2007)

hit	Umebayashi K (1999)

Phenotypic Enhancement

hit	Schuldiner M (2005)

YER089C

Ptc2p|Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation; protein coding

Gene Ontology: [View List]

Reconstituted Complex

hit	Welihinda AA (1998)

hit	Welihinda AA (1998)

Biochemical Activity

bait	Welihinda AA (1998)

ADA4

SWI9

YGR252W

Gcn5p|Histone acetyltransferase, acetylates N-terminal lysines on histones H2B and H3; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; founding member of the Gcn5p-related N-acetyltransferase superfamily; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

bait	Welihinda AA (1997)

Reconstituted Complex

bait	Welihinda AA (1997)

hit	Welihinda AA (1997)

YLR361C

Dcr2p|Phosphoesterase involved in downregulation of the unfolded protein response, at least in part via dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

bait	Guo J (2006)

Biochemical Activity

bait	Guo J (2006)

Synthetic Lethality

bait	Guo J (2006)

ERN1

YHR079C

Ire1p|Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Papa FR (2003)

Affinity Capture-Western

hit	Oikawa D (2007)

YPR181C

GTPase-activating protein; component of the Sec23p-Sec24p heterodimeric complex of the COPII vesicle coat, involved in ER to Golgi transport and autophagy; stimulates the GDP-bound form of Sar1p|Sec23p; protein coding

Gene Ontology: [View List]

bait	Higashio H (2002)
bait	Sato M (2002)

Phenotypic Enhancement

hit	Taxis C (2002)

SED2

YNR026C

Guanine nucleotide exchange factor (GEF); glycosylated integral membrane protein of the endoplasmic reticulum, important for the initiation of transport vesicle budding from the endoplasmic reticulum through activation of the GTPase Sar1p|Sec12p; protein coding

Gene Ontology: [View List]

bait	Higashio H (2002)
bait	Sato M (2002)

Synthetic Growth Defect

hit	Chang HJ (2004)

ANU3

YLR208W

Component of both the Nup84 nuclear pore sub-complex and of the COPII complex (Sar1p, Sec13p, Sec16p, Sec23p, Sec24p, Sec31p, Sfb2p, and Sfb3p) which is important for the formation of ER to Golgi transport vesicles|Sec13p; protein coding

Gene Ontology: [View List]

bait	Higashio H (2002)
bait	Sato M (2002)

Synthetic Growth Defect

hit	Chang HJ (2004)

ERN4

IRE15

YFL031W

Hac1p|bZIP transcription factor (ATF/CREB1 homolog) that regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway utilizing Ire1p, Trl1p and Ada5p facilitates efficient Hac1p synthesis; protein coding

Gene Ontology: [View List]

hit	Niwa M (2005)

Synthetic Rescue

hit	Nikawa J (1996)

LPF1

YPL085W

COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p|Sec16p; protein coding

Gene Ontology: [View List]

Synthetic Growth Defect

hit	Chang HJ (2004)

bait	Sato M (2002)

UBC7

YMR022W

Ubc7p|Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly; protein coding

Gene Ontology: [View List]

Synthetic Lethality

bait	Friedlander R (2000)

Phenotypic Enhancement

hit	Schuldiner M (2005)

CER1

SSI1

YKL073W

Lhs1p|Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; member of the Hsp70 family; localizes to the lumen of the ER; regulated by the unfolded protein response pathway; protein coding

Gene Ontology: [View List]

Synthetic Lethality

bait	Tyson JR (2000)

Phenotypic Enhancement

hit	Schuldiner M (2005)

YMR229C

Protein required for the synthesis of both 18S and 5.8S rRNA; C-terminal region is crucial for the formation of 18S rRNA and N-terminal region is required for the 5.8S rRNA; component of small ribosomal subunit (SSU) processosome|Rrp5p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YBR285W

Putative protein of unknown function; YBR285W is not an essential gene and deletion of YBR285W leads to poor growth on glucose-minimal medium at 15C|Ybr285wp; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

ADD37

Add37p|Protein of unknown function involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YLL043W

Fps1p|Plasma membrane glycerol channel, member of the major intrinsic protein (MIP) family of channel proteins; involved in efflux of glycerol and in uptake of the trivalent metalloids arsenite and antimonite; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YMK1

YNL074C

Mlf3p|Serine-rich protein of unknown function; overproduction suppresses the growth inhibition caused by exposure to the immunosuppressant leflunomide; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YML037C

Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene|Yml037cp; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

AIM3

Aim3p|Protein interacting with Rsv167p; null mutant results in Altered rate of mitochondrial loss and severe growth defect in minimal glycerol media; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YJL108C

Pheromone-regulated protein, predicted to have 5 transmembrane segments|Prm10p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

HPH2

YAL028W

Frt2p|Tail-anchored endoplasmic reticulum membrane protein, interacts with homolog Frt1p but is not a substrate of calcineurin (unlike Frt1p), promotes growth in conditions of high Na+, alkaline pH, or cell wall stress; potential Cdc28p substrate; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YLR006C

Cytoplasmic response regulator, part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate|Ssk1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YPR067W

Isa2p|Protein required for maturation of mitochondrial and cytosolic Fe/S proteins, localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YMR140W

Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase|Sip5p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

UTP3

YDL153C

Component of the small (ribosomal) subunit (SSU) processosome required for pre-18S rRNa processing; essential nucleolar protein that, when overproduced, disrupts silencing|Sas10p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YBL007C

Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains|Sla1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YOR113W

Azf1p|Zinc-finger transcription factor, involved in induction of CLN3 transcription in response to glucose; genetic and physical interactions indicate a possible role in mitochondrial transcription or genome maintenance; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YNR047W

Putative protein kinase that, when overexpressed, interferes with pheromone-induced growth arrest; localizes to the cytoplasm; potential Cdc28p substrate|Ynr047wp; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YDR120C

Trm1p|tRNA methyltransferase, localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YDR388W

Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin|Rvs167p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YPR158C-C

Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag|Ypr158c-cp; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

HAL7

YDR259C

Putative basic leucine zipper (bZIP) transcription factor; overexpression increases sodium and lithium tolerance|Yap6p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

SQS1

Protein of unknown function; overexpression antagonizes the suppression of splicing defects by spp382 mutants; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus|Sqs1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YPL257W-A

Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag|Ypl257w-ap; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YNL054W-A

Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag|Ynl054w-ap; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YER122C

ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p|Glo3p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

CVT19

YOL082W

Atg19p|Protein involved in the cytoplasm-to-vacuole targeting pathway and in autophagy, recognizes cargo proteins and delivers them to the preautophagosomal structure for eventual engulfment by the autophagosome and degradation; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

PDR2

YOR162C

Yrr1p|Zn2-Cys6 zinc-finger transcription factor that activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

KRE28

Protein of unknown function that localizes to the nuclear side of the spindle pole body and along short spindles; deletion mutants have short telomeres; forms a complex with Spc105p|Ydr532cp; protein coding

Gene Ontology: