| Name |
Aliases |
 |
Description |
| Evidence Code |
| Role |
Source [Interactions] |
|
|
|
| UBP3 |
YER151C |
|
Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde transport between endoplasmic reticulum and Golgi compartments; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin
|
|
|
|
|
| GET1 |
MDM39YGL020C |
|
Subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance
|
|
|
|
|
| GET2 |
RMD7HUR2YER083C |
|
Subunit of the GET complex; required for meiotic nuclear division and for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; may be involved in cell wall function
|
|
|
|
|
| RIC1 |
YLR039C |
|
Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
|
|
|
|
|
| CSM3 |
YMR048W |
|
Protein required for accurate chromosome segregation during meiosis
|
|
|
|
|
| BRE5 |
YNR051C |
|
Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
|
|
|
|
|
| TOP1 |
MAK17MAK1YOL006C |
|
Topoisomerase I, nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination
|
|
|
|
|
| YPT6 |
YLR262C |
|
GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
|
|
|
|
|
| BIM1 |
YEB1YER016W |
|
Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
|
|
|
|
|
| BNI4 |
YNL233W |
|
Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p
|
|
|
|
|
| LSM1 |
SPB8YJL124C |
|
Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs
|
|
|
|
|
| SMI1 |
KNR4YGR229C |
|
Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
|
|
|
|
|
| CKB2 |
YOR039W |
|
Beta' regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB1, the many substrates include transcription factors and all RNA polymerases
|
|
|
|
|
| DST1 |
PPR2YGL043W |
|
General transcription elongation factor TFIIS, enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites
|
|
|
|
|
| SOH1 |
MED31YGL127C |
|
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
|
|
|
|
|
| VPS72 |
SWC2YDR485C |
|
Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
|
|
|
|
|
| SWR1 |
YDR334W |
|
Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
|
|
|
|
|
| POL32 |
YJR043C |
|
Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
|
|
|
|
|
| HTZ1 |
HTA3YOL012C |
|
Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
|
|
|
|
|
| FKS1 |
CWH53GSC1PBR1YLR342W |
|
Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
|
|
|
|
|
| SAP155 |
YFR040W |
|
Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p
|
|
|
|
|
| ELP2 |
TOT2YGR200C |
|
Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
|
|
|
|
|
| SPF1 |
COD1PER9PIO1YEL031W |
|
P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1
|
|
|
|
|
| PHO80 |
VAC5AGS3TUP7YOL001W |
|
Cyclin, negatively regulates phosphate metabolism; Pho80p-Pho85p (cyclin-CDK complex) phosphorylates Pho4p and Swi5p; deletion of PHO80 leads to aminoglycoside supersensitivity; truncated form of PHO80 affects vacuole inheritance
|
|
|
|
|
| SCS7 |
FAH1YMR272C |
|
Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
|
|
|
|
|
| DOA1 |
UFD3ZZZ4YKL213C |
|
WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase
|
|
|
|
|
| SEC28 |
ANU2YIL076W |
|
Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth
|
|
|
|
|
| GSG1 |
MUM1TRS85YDR108W |
|
Subunit of TRAPP (transport protein particle), a multi-subunit complex involved in targeting and/or fusion of ER-to-Golgi transport vesicles with their acceptor compartment; protein has late meiotic role, following DNA replication
|
|
|
|
|
| MNN10 |
BED1SLC2REC41YDR245W |
|
Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family
|
|
|
|
|
| MCK1 |
YPK1YNL307C |
|
Protein serine/threonine/tyrosine (dual-specificity) kinase involved in control of chromosome segregation and in regulating entry into meiosis; related to mammalian glycogen synthase kinases of the GSK-3 family
|
|
|
|
|
| GIS2 |
YNL255C |
|
Protein with seven cysteine-rich CCHC zinc-finger motifs, similar to human CNBP, proposed to be involved in the RAS/cAMP signaling pathway
|
|
|
|
|
| SRO9 |
YCL037C |
|
Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif
|
|
|
|
|
| MED8 |
YBR193C |
|
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
|
|
|
|
|
| NUP84 |
YDL116W |
|
Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis
|
|
|
|
|
| SPT4 |
YGR063C |
|
Protein that forms a complex with Spt5p and mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing; in addition, Spt4p is involved in kinetochore function and gene silencing
|
|
|
|
|
| UME6 |
NIM2CAR80RIM16YDR207C |
|
Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p
|
|
|
|
|
| SAP190 |
YKR028W |
|
Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p
|
|
|
|
|
| THP2 |
YHR167W |
|
Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
|
|
|
|
|
| BUD27 |
URI1YFL023W |
|
Protein involved in bud-site selection, nutrient signaling, and gene expression controlled by the TOR kinase; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
|
|
|
|
|
| PAT1 |
MRT1YCR077C |
|
Topoisomerase II-associated deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; functionally linked to Pab1p
|
|
|
|
|
| BMH1 |
APR6YER177W |
|
14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling
|
|
|
|
|
| IWR1 |
YDL115C |
|
Protein of unknown function, deletion causes hypersensitivity to the K1 killer toxin
|
|
|
|
|
| SEC22 |
TSL26SLY2YLR268W |
|
R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
|
|
|
|
|
| SIR4 |
STE9ASD1UTH2YDR227W |
|
Silent information regulator that, together with SIR2 and SIR3, is involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan
|
|
|
|
|
| LSM6 |
YDR378C |
|
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
|
|
|
|
|
| OPI3 |
PEM2YJR073C |
|
Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis
|
|
|
|
|
| INP53 |
SJL3SOP2YOR109W |
|
Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
|
|
|
|
|
| CLN3 |
WHI1FUN10DAF1YAL040C |
|
G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis
|
|
|
|
|
| STE50 |
YCL032W |
|
Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
|
|
|
|
|
| TFP1 |
VMA1CLS8YDL185W |
|
Vacuolar ATPase V1 domain subunit A containing the catalytic nucleotide binding sites; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
|
|
|
|
|
| UBC13 |
YDR092W |
|
Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
|
|
|
|
|
| SLA1 |
YBL007C |
|
Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
|
|
|
|
|
| SHP1 |
UBX1YBL058W |
|
UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate
|
|
|
|
|
| RPA34 |
YJL148W |
|
RNA polymerase I subunit A34.5
|
|
|
|
|
| SUB1 |
TSP1YMR039C |
|
Transcriptional coactivator, facilitates elongation by influencing enzymes that modify RNAP II, acts in a peroxide resistance pathway involving Rad2p; suppressor of TFIIB mutations
|
|
|
|
|
| CLG1 |
YGL215W |
|
Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
|
|
|
|
|
| MUB1 |
YMR100W |
|
Protein of unknown function, deletion causes multi-budding phenotype; has similarity to Aspergillus nidulans samB gene
|
|
|
|
|
| VPS8 |
VPT8FUN15YAL002W |
|
Membrane-associated protein that interacts with Vps21p to facilitate soluble vacuolar protein localization; component of the CORVET complex; required for localization and trafficking of the CPY sorting receptor; contains RING finger motif
|
|
|
|
|
| CAC2 |
YML102W |
|
Component of the chromatin assembly complex (with Rlf2p and Msi1p) that assembles newly synthesized histones onto recently replicated DNA, required for building functional kinetochores, conserved from yeast to humans
|
|
|
|
|
| SKY1 |
YMR216C |
|
SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
|
|
|
|
|
| BEM1 |
SRO1YBR200W |
|
Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
|
|
|
|
|
| RXT2 |
RAF60YBR095C |
|
Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth
|
|
|
|
|
| SRB2 |
HRS2MED20YHR041C |
|
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
|
|
|
|
|
| VPS30 |
ATG6VPT30APG6YPL120W |
|
Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; ortholog of the higher eukaryotic gene Beclin 1
|
|
|
|
|
| SEC14 |
PIT1YMR079W |
|
Phosphatidylinositol/phosphatidylcholine transfer protein involved in coordinate regulation of PtdIns and PtdCho metabolism, products of which are regulators in Golgi to plasma membrane transport; functionally homologous to mammalian PITPs
|
|
|
|
|
| SWI4 |
ART1YER111C |
|
DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair
|
|
|
|
|
| MNN11 |
YJL183W |
|
Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
|
|
|
|
|
| IRA2 |
CCS1GLC4YOL081W |
|
GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin
|
|
|
|
|
| ACS2 |
YLR153C |
|
Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
|
|
|
|
|
| SIN4 |
GAL22SDI3RYE1YNL236W |
|
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
|
|
|
|
|
| RXT3 |
YDL076C |
|
Subunit of the RPD3L complex; involved in histone deacetylation
|
|
|
|
|
| BRE2 |
CPS60YLR015W |
|
Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; involved in telomere maintenance; similar to trithorax-group protein ASH2L
|
|
|
|
|
| RET2 |
YFR051C |
|
Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
|
|
|
|
|
| ELG1 |
RTT110YOR144C |
|
Protein required for S phase progression and telomere homeostasis, forms an alternative replication factor C complex important for DNA replication and genome integrity; involved in homologous recombination-mediated DNA repair
|
|
|
|
|
| SWC5 |
AOR1YBR231C |
|
Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
|
|
|
|
|
| ORC2 |
SIR5RRR1YBR060C |
|
Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p
|
|
|
|
|
| HSP82 |
HSP90YPL240C |
|
Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding and nucleotide addition; interacts with Cns1p, Cpr6p, Cpr7p, Sti1p
|
|
|
|
|
| CTI6 |
RXT1YPL181W |
|
Protein that relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
|
|
|
|
|
| GIM4 |
PFD2YEL003W |
|
Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
|
|
|
|
|
| YAF9 |
YNL107W |
|
Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain
|
|
|
|
|
| ELP3 |
TOT3HPA1YPL086C |
|
Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin
|
|
|
|
|
| CTF4 |
CHL15POB1YPR135W |
|
Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
|
|
|
|
|
| SET2 |
EZL1YJL168C |
|
Histone methyltransferase with a role in transcriptional elongation, methylates a lysine residue of histone H3; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
|
|
|
|
|
| CHS3 |
CSD2KTI2DIT101YBR023C |
|
Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
|
|
|
|
|
| HOC1 |
YJR075W |
|
Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
|
|
|
|
|
| ERV14 |
YGL054C |
|
Protein localized to COPII-coated vesicles, involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon
|
|
|
|
|
| ACT1 |
ABY1END7YFL039C |
|
Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
|
|
|
|
|
| SKT5 |
CSD4CAL2CHS4YBL061C |
|
Activator of Chs3p (chitin synthase III), recruits Chs3p to the bud neck via interaction with Bni4p; has similarity to Shc1p, which activates Chs3p during sporulation
|
|
|
|
|
| SKI3 |
SKI5YPR189W |
|
Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski2p and Ski8p; required for repressing propagation of dsRNA viruses
|
|
|
|
|
| RGP1 |
YDR137W |
|
Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
|
|
|
|
|
| SEC27 |
YGL137W |
|
Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
|
|
|
|
|
| THI6 |
YPL214C |
|
Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities, required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
|
|
|
|
|
| GET3 |
ARR4YDL100C |
|
ATPase, subunit of the GET complex; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion; involved in resistance to heat and metal stress
|
|
|
|
|
| GYP1 |
YOR29-21YOR070C |
|
Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
|
|
|
|
|
| UBP6 |
YFR010W |
|
Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds
|
|
|
|
|
| RRP5 |
YMR229C |
|
Protein required for the synthesis of both 18S and 5.8S rRNA; C-terminal region is crucial for the formation of 18S rRNA and N-terminal region is required for the 5.8S rRNA; component of small ribosomal subunit (SSU) processosome
|
|
|
|
|
| STM1 |
MPT4YLR150W |
|
Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations
|
|
|
|
|
| NIP1 |
YMR309C |
|
Subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
|
|
|
|
|
| NOP2 |
YNA1YNL061W |
|
Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles
|
|
|
|
|
| YGR054W |
YGR054W |
|
Eukaryotic initiation factor (eIF) 2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A
|
|
|
|
|
| IDP1 |
YDL066W |
|
Mitochondrial NADP-specific isocitrate dehydrogenase, catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
|
|
|
|
|
| SGV1 |
BUR1YPR161C |
|
Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p, which is the largest subunit of RNA polymerase II; regulated by Cak1p
|
|
|
|
|
| SPT5 |
YML010W |
|
Protein that forms a complex with Spt4p and mediates both activation and inhibition of transcription elongation; Spt4p-Spt5p complex also plays a role in pre-mRNA processing
|
|
|
|
|
| HYP2 |
YEL034W |
|
Translation initiation factor eIF-5A, promotes formation of the first peptide bond; similar to and functionally redundant with Anb1p; possible role in translation elongation; undergoes an essential hypusination modification
|
|
|
|
|
| YAR010C |
YAR010C |
|
Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YARCTY1-1 TYB is mutant and probably non-functional
|
|
|
|
|
| GCN2 |
AAS1YDR283C |
|
Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex
|
|
|
|
|
| MSC7 |
YHR039C |
|
Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
|
|
|
|
|
| RIM1 |
YCR028C-A |
|
Single-stranded DNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication
|
|
|
|
|
| TAF13 |
TAF19FUN81YML098W |
|
TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
|
|
|
|
|
| YDR415C |
YDR415C |
|
Putative protein of unknown function
|
|
|
|
|
| YRA1 |
SHE11YDR381W |
|
Nuclear protein that binds to RNA and to Mex67p, required for export of poly(A)+ mRNA from the nucleus; member of the REF (RNA and export factor binding proteins) family; another family member, Yra2p, can substitute for Yra1p function
|
|
|
|
|
| DED1 |
SPP81YOR204W |
|
ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility
|
|
|
|
|
| UTP5 |
YDR398W |
|
Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
|
|
|
|
|
| UBI4 |
SCD2UB14YLL039C |
|
Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats
|
|
|
|
|
| TAP42 |
YMR028W |
|
Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
|
|
|
|
|
| TAF7 |
TAF67YMR227C |
|
TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation
|
|
|
|
|
| LDB18 |
YLL049W |
|
Protein of unknown function; null mutant shows a reduced affinity for the alcian blue dye suggesting a decreased net negative charge of the cell surface
|
|
|
|
|
| TFA2 |
YKR062W |
|
TFIIE small subunit, involved in RNA polymerase II transcription initiation
|
|
|
|
|
| PRP16 |
YKR086W |
|
RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity
|
|
|
|
|
| NUP188 |
YML103C |
|
Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p
|
|
|
|
|
| RCO1 |
YMR075W |
|
Essential subunit of the histone deacetylase Rpd3S complex; interacts with Eaf3p
|
|
|
|
|
| LSM3 |
SMX4USS2YLR438C-A |
|
Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
|
|
|
|
|
| SAS2 |
YMR127C |
|
Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
|
|
|
|
|
| HLJ1 |
YMR161W |
|
Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Hdj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
|
|
|
|
|
| ADH6 |
ADHVIYMR318C |
|
NADPH-dependent medium chain alcohol dehydrogenase with broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance
|
|
|
|
|
| CDC3 |
YLR314C |
|
Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM
|
|
|
|
|
| SSL1 |
YLR005W |
|
Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
|
|
|
|
|
| RPD3 |
SDI2MOF6REC3YNL330C |
|
Histone deacetylase; regulates transcription and silencing
|
|
|
|
|
| MVP1 |
YMR004W |
|
Protein required for sorting proteins to the vacuole; overproduction of Mvp1p suppresses several dominant VPS1 mutations; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole
|
|
|
|
|
| FYV7 |
YLR068W |
|
Protein of unknown function, required for survival upon exposure to K1 killer toxin; involved in processing the 35S rRNA primary transcript to generate the 20S and 27SA2 pre-rRNA transcripts
|
|
|
|
|
| POB3 |
YML069W |
|
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p), which facilitates RNA Pol II transcription elongation through nucleosomes by destabilizing and then reassembling nucleosome structure; interacts with DNA polymerase alpha (Pol1p)
|
|
|
|
|
| YLR152C |
YLR152C |
|
Putative protein of unknown function; YLR152C is not an essential gene
|
|
|
|
|
| RPL8B |
KRB1SCL41YLL045C |
|
Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has similarity to rat L7a ribosomal protein; mutation results in decreased amounts of free 60S subunits
|
|
|
|
|
| SYM1 |
YLR251W |
|
Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17
|
|
|
|
|
| FMS1 |
YMR020W |
|
Polyamine oxidase, converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
|
|
|
|
|
| SFI1 |
YLL003W |
|
Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C
|
|
|
|
|
| SST2 |
YLR452C |
|
GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
|
|
|
|
|
| HTD2 |
RMD12YHR067W |
|
Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
|
|
|
|
|
| ARC1 |
YGL105W |
|
Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids
|
|
|
|
|
| SCS3 |
FIT2BYGL126W |
|
Protein required for inositol prototrophy, identified as an ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis; disputed role in the synthesis of inositol phospholipids from inositol
|
|
|
|
|
| CEG1 |
YGL130W |
|
Alpha (guanylyltransferase) subunit of the mRNA capping enzyme, a heterodimer (the other subunit is CET1, an RNA 5'-triphophatase) involved in adding the 5' cap to mRNA; the mammalian enzyme is a single bifunctional polypeptide
|
|
|
|
|
| HUR1 |
YGL168W |
|
Protein required for hydroxyurea resistance; has possible roles in DNA replication and maintenance of proper telomere length
|
|
|
|
|
| DUO1 |
YGL061C |
|
Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
|
|
|
|
|
| CKB1 |
YGL019W |
|
Beta regulatory subunit of casein kinase 2, a Ser/Thr protein kinase with roles in cell growth and proliferation; the holoenzyme also contains CKA1, CKA2 and CKB2, the many substrates include transcription factors and all RNA polymerases
|
|
|
|
|
| PHB1 |
YGR132C |
|
Subunit of the prohibitin complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation
|
|
|
|
|
| NUP57 |
YGR119C |
|
Nucleoporin, essential subunit of the nuclear pore complex (NPC), functions as the organizing center of an NPC subcomplex containing Nsp1p, Nup49p, Nup57p, and Nic96p
|
|
|
|
|
| PIL1 |
YGR086C |
|
Primary component of eisosomes, which are large immobile cell cortex structures associated with endocytosis; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria
|
|
|
|
|
| VAM7 |
VPS43YGL212W |
|
Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; functions with a syntaxin homolog Vam3p in vacuolar protein trafficking
|
|
|
|
|
| RIM15 |
TAK1YFL033C |
|
Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
|
|
|
|
|
| GPI8 |
YDR331W |
|
ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog
|
|
|
|
|
| SRP101 |
YDR292C |
|
Signal recognition particle (SRP) receptor - alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with SRP102p
|
|
|
|
|
| PMP3 |
SNA1YDR276C |
|
Small plasma membrane protein related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature, not essential for viability, deletion causes hyperpolarization of the plasma membrane potential
|
|
|
|
|
| PRP28 |
YDR243C |
|
RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
|
|
|
|
|
| MSN5 |
KAP142STE21YDR335W |
|
Karyopherin involved in nuclear import and export; shown to be responsible for nuclear import of replication protein A and for export of several proteins including Swi6p, Far1p, and Pho4p; cargo dissociation involves binding to RanGTP
|
|
|
|
|
| PAC11 |
YDR488C |
|
Dynein intermediate chain, acts in the cytoplasmic dynein pathway, forms cortical cytoplasmic microtubule capture site with Num1p; null mutant is defective in nuclear migration, essential in the absence of CIN8
|
|
|
|
|
| COG3 |
SEC34GRD20YER157W |
|
Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
|
|
|
|
|
| GLE2 |
RAE1YER107C |
|
Component of the nuclear pore complex required for polyadenylated RNA export but not for protein import, homologous to S. pombe Rae1p
|
|
|
|
|
| WBP1 |
YEL002C |
|
Beta subunit of the oligosaccharyl transferase (OST) glycoprotein complex; required for N-linked glycosylation of proteins in the endoplasmic reticulum
|
|
|
|
|
| CLC1 |
SCD4YGR167W |
|
Clathrin light chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function, two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component
|
|
|
|
|
| PBP1 |
MRS16YGR178C |
|
Protein interacting with poly(A)-binding protein Pab1p; likely involved in controlling the extent of mRNA polyadenylation; forms a complex with Mkt1p that may regulate HO translation; interacts with Lsm12p in a copurification assay
|
|
|
|
|
| TOR1 |
DRR1YJR066W |
|
PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis
|
|
|
|
|
| CDC8 |
YJR057W |
|
Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
|
|
|
|
|
| MTR4 |
DOB1YJL050W |
|
Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA
|
|
|
|
|
| GWT1 |
YJL091C |
|
Protein involved in the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) to form glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
|
|
|
|
|
