BioGRID | Network Layout for PHO80 for Saccharomyces cerevisiae

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PHO80

Saccharomyces cerevisiae

Aliases: AGS3, TUP7, VAC5, YOL001W

Description: Cyclin, negatively regulates phosphate metabolism; Pho80p-Pho85p (cyclin-CDK complex) phosphorylates Pho4p and Swi5p; deletion of PHO80 leads to aminoglycoside supersensitivity; truncated form of PHO80 affects vacuole inheritance|Pho80p; protein coding

Links: [Entrez-Gene] [GeneDB] [Germ Online] [SGD]

Gene Ontology: 4 total GO annotations for this record. [view list]

Download interactions associated with PHO80

Name

Aliases

Description

Evidence Code(s)

Role

Source(s)

YGR233C

Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p|Pho81p; protein coding

Gene Ontology: [View List]

Reconstituted Complex

bait	Hirst K (1994)
bait	Huang S (2001)
hit	Huang K (2007)
bait	Lee YS (2008)

bait	Lee M (2000)

Affinity Capture-Western

hit	Knight JP (2004)
hit	Waters NC (2004)
hit	Huang S (2001)
bait	Schneider KR (1994)

Affinity Capture-MS

bait	Krogan NJ (2006)
bait	Gavin AC (2006)
bait	Collins SR (2007)

Biochemical Activity

hit	Dephoure N (2005)

Synthetic Rescue

bait	Lau WW (1998)

Phenotypic Suppression

bait	Creasy CL (1993)
bait	Swinnen E (2005)

YFR034C

Basic helix-loop-helix (bHLH) transcription factor of the myc-family; binds cooperatively with Pho2p to the PHO5 promoter; function is regulated by phosphorylation at multiple sites and by phosphate availability|Pho4p; protein coding

Gene Ontology: [View List]

bait	Lee M (2000)
bait	Jayaraman PS (1994)

Affinity Capture-Western

hit	Waters NC (2004)

Reconstituted Complex

bait/hit	Byrne M (2004)
hit	Huang K (2007)

Biochemical Activity

hit	Byrne M (2004)
hit	Jeffery DA (2001)
hit	Dephoure N (2005)
hit	Ptacek J (2005)

Synthetic Rescue

hit	Huang D (2002)

Phenotypic Suppression

bait	Yoshida K (1989)
hit	Wickert S (1998)
bait	Okada H (1992)
hit	Holbein S (2008)

LDB15

YPL031C

Cyclin-dependent kinase, with ten cyclin partners; involved in environmental stress response; in phosphate-rich conditions, Pho85p-Pho80p complex phosphorylates Pho4p which in turn represses PHO5|Pho85p; protein coding

Gene Ontology: [View List]

Affinity Capture-Western

bait	Kaffman A (1994)
bait	Waters NC (2004)

bait	Lee M (2000)
bait	Santos RC (1995)

Reconstituted Complex

bait	Santos RC (1995)
bait	Waters NC (2004)

Biochemical Activity

bait	Waters NC (2004)

Affinity Capture-MS

bait	Gavin AC (2006)
hit	Collins SR (2007)

Co-crystal Structure

bait	Huang K (2007)

bait	Madden SL (1990)
hit	Gilliquet V (1990)

YLR410W

Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of IP7 by Vip1p is important for phosphate signaling; likely involved in cortical actin cytoskeleton function, by analogy with S. pombe ortholog asp1|Vip1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Dephoure N (2005)
hit	Ptacek J (2005)

YMR311C

Glc8p|Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Dephoure N (2005)
hit	Ptacek J (2005)

PAH1

YMR165C

Mg2+-dependent phosphatidate (PA) phosphatase, catalyzes the dephosphorylation of PA to yield diacylglycerol and Pi, responsible for de novo lipid synthesis; homologous to mammalian lipin 1|Pah1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Dephoure N (2005)
hit	Ptacek J (2005)

YLR262C

GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6|Ypt6p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

Phenotypic Enhancement

hit	Collins SR (2007)

CHL15

POB1

YPR135W

Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion|Ctf4p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

Synthetic Growth Defect

bait	Pan X (2006)

SON1

UFD5

YDL020C

Rpn4p|Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; protein coding

Gene Ontology: [View List]

Synthetic Growth Defect

bait	Pan X (2006)

Phenotypic Enhancement

hit	Collins SR (2007)

YML123C

High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p|Pho84p; protein coding

Gene Ontology: [View List]

Synthetic Rescue

hit	Holbein S (2008)

Phenotypic Suppression

hit	Holbein S (2008)

YJL060W

Arylformamidase, involved in biosynthesis of nicotinic acid from tryptophan via kynurenine pathway; potential Cdc28p substrate|Bna3p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YMR276W

Dsk2p|Nuclear-enriched ubiquitin-like polyubiquitin-binding protein, required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle, involved in proteolysis, interacts with the proteasome; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YNL136W

Eaf7p|Subunit of the NuA4 histone acetyltransferase complex, which acetylates the N-terminal tails of histones H4 and H2A; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YLR102C

Apc9p|Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YLL018C-A

Cox19p|Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YER089C

Ptc2p|Type 2C protein phosphatase; dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; role in DNA checkpoint inactivation; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

ECM6

YML048W

ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression|Gsf2p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

MUT5

YER095W

Rad51p|Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YJR129C

Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm|Yjr129cp; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YDL201W

Subunit of a tRNA methyltransferase complex composed of Trm8p and Trm82p that catalyzes 7-methylguanosine modification of tRNA|Trm8p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YKL127W

Pgm1p|Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YBR109C

Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin|Cmd1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YEL042W

Gda1p|Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YPL146C

Nop53p|Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

PBY1

Pby1p|Putative tubulin tyrosine ligase associated with P-bodies; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

WTM3

YPL139C

Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p|Ume1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YNL007C

Sis1p|Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; shares similarity with bacterial DnaJ proteins; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YLR146C

Spe4p|Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YNT20

YLR059C

RNA exonuclease, required for U4 snRNA maturation; functions redundantly with Rnh70p in 5.8S rRNA maturation, and with Rnh70p and Rex3p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases|Rex2p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

ACK1

Ack1p|Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

GPP1

YIL053W

Constitutively expressed isoform of DL-glycerol-3-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and, along with the Hor2p/Gpp2p isoform, osmotic stress|Rhr2p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YDR169C

Protein that binds Sin3p in a two-hybrid assay|Stb3p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YDR256C

Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation|Cta1p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YFR010W

Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; deletion causes hypersensitivity to cycloheximide and other toxic compounds|Ubp6p; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

YMR153W

Nup53p|Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex; protein coding

Gene Ontology: [View List]

Biochemical Activity

hit	Ptacek J (2005)

MMD1

YJR070C

Deoxyhypusine hydroxylase, a HEAT-repeat containing metalloenzyme that catalyses hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning|Lia1p; protein coding

Gene Ontology: [View List]