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BioGRID | Network Layout for SPC34 for Saccharomyces cerevisiae
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SPC34

 Saccharomyces cerevisiae
The BioGRID Database Seperator
Aliases: YKR037C
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Description: Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Links: [UniprotKB] | [SGD] | [Entrez Gene] | [GeneDB]
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Gene Ontology: 16 total GO annotations for this record. [view list]
The BioGRID Database Seperator
PIXEL
The BioGRID Database Seperator
Download interactions associated with SPC34

SPC34 was identified with 87 protein interactions and 19 genetic interactions
Displaying:
 Name Aliases The BioGRID Database Seperator Description
Evidence Code
Role Source [Interactions]
The BioGRID Database Seperator
 SPC19
  • YDR201W
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
    Gene Ontology: [View List]
    Two-hybrid
    hit Ito T (2001) [1585]
    hit Uetz P (2000) [875]
    hit Ikeuchi A (2003) [4]
    bait/hit Wong J (2007) [604]
    Affinity Capture-MS
    hit Janke C (2002) [15]
    bait Krogan NJ (2006) [7079]
    bait Collins SR (2007) [9064]
    Reconstituted Complex
    bait/hit Shang C (2003) [35]
    Co-fractionation
    bait De Wulf P (2003) [64]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DAD1
  • YDR016C
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
    Gene Ontology: [View List]
    Two-hybrid
    hit Ito T (2001) [1585]
    hit Ikeuchi A (2003) [4]
    hit Wong J (2007) [604]
    Affinity Capture-MS
    bait Cheeseman IM (2002) [28]
    bait Janke C (2002) [15]
    bait Krogan NJ (2006) [7079]
    bait Collins SR (2007) [9064]
    Affinity Capture-Western
    bait Cheeseman IM (2001) [8]
    Co-purification
    bait/hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DAM1
  • YGR113W
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
    Gene Ontology: [View List]
    Two-hybrid
    bait Ito T (2001) [1585]
    bait Shang C (2003) [35]
    hit Ikeuchi A (2003) [4]
    hit Wong J (2007) [604]
    Reconstituted Complex
    bait/hit Shang C (2003) [35]
    Co-fractionation
    hit De Wulf P (2003) [64]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DUO1
  • YGL061C
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
    Gene Ontology: [View List]
    Two-hybrid
    bait/hit Ito T (2001) [1585]
    hit Ikeuchi A (2003) [4]
    bait/hit Wong J (2007) [604]
    Affinity Capture-Western
    hit Li Y (2002) [12]
    Reconstituted Complex
    bait/hit Shang C (2003) [35]
    Affinity Capture-MS
    bait Krogan NJ (2006) [7079]
    bait Collins SR (2007) [9064]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ASK1
  • YKL052C
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases
    Gene Ontology: [View List]
    Affinity Capture-Western
    bait Li Y (2002) [12]
    Affinity Capture-MS
    bait Li JM (2005) [22]
    bait Krogan NJ (2006) [7079]
    bait Collins SR (2007) [9064]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     HSK3
  • YKL138C-A
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
    Gene Ontology: [View List]
    Co-purification
    bait Westermann S (2005) [9]
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    Affinity Capture-MS
    hit Krogan NJ (2006) [7079]
    Two-hybrid
    bait Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DAD2
  • HSK1
  • YKR083C
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Janke C (2002) [15]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    Two-hybrid
    hit Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DAD4
  • HSK2
  • YDR320C-A
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006) [7079]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    Two-hybrid
    bait Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     DAD3
  • YBR233W-A
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
    Gene Ontology: [View List]
    Co-purification
    hit Miranda JJ (2005) [18]
    hit Gestaut DR (2008) [9]
    hit Miranda JJ (2007) [11]
    Two-hybrid
    bait Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SLI15
  • YBR156C
    		•  The BioGRID Database Seperator Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase
    Gene Ontology: [View List]
    Two-hybrid
    bait/hit Wong J (2007) [604]
    Biochemical Activity
    bait Nakajima Y (2009) [20]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     ATG17
  • APG17
  • YLR423C
    		•  The BioGRID Database Seperator Scaffold potein responsible for pre-autophagosomal structure organization; interacts with and is required for activation of Apg1p protein kinase; involved in autophagy but not in the Cvt (cytoplasm to vacuole targeting) pathway
    Gene Ontology: [View List]
    Two-hybrid
    hit Ito T (2001) [1585]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     BIM1
  • YEB1
  • YER016W
    		•  The BioGRID Database Seperator Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally
    Gene Ontology: [View List]
    Two-hybrid
    hit Wong J (2007) [604]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     IPL1
  • PAC15
  • YPL209C
    		•  The BioGRID Database Seperator Aurora kinase involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; associates with Sli15p, which promotes Ipl1p association with mitotic spindle
    Gene Ontology: [View List]
    Biochemical Activity
    bait Nakajima Y (2009) [20]
    Synthetic Growth Defect
    hit Cheeseman IM (2002) [28]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CCT3
  • BIN2
  • TCP3
  • YJL014W
    		•  The BioGRID Database Seperator Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Dekker C (2008) [179]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SHE1
  • YBL031W
    		•  The BioGRID Database Seperator Cytoskeletal protein of unknown function; overexpression causes growth arrest
    Gene Ontology: [View List]
    Two-hybrid
    hit Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     STU2
  • YLR045C
    		•  The BioGRID Database Seperator Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p
    Gene Ontology: [View List]
    Two-hybrid
    bait/hit Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SPC25
  • YER018C
    		•  The BioGRID Database Seperator Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
    Gene Ontology: [View List]
    Two-hybrid
    hit Wong J (2007) [604]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     JSN1
  • PUF1
  • YJR091C
    		•  The BioGRID Database Seperator Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells
    Gene Ontology: [View List]
    Two-hybrid
    hit Uetz P (2000) [875]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     GCN1
  • YGL195W
    		•  The BioGRID Database Seperator Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA
    Gene Ontology: [View List]
    Affinity Capture-MS
    hit Krogan NJ (2006) [7079]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SPC110
  • NUF1
  • XCM1
  • YDR356W
    		•  The BioGRID Database Seperator Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
    Gene Ontology: [View List]
    Co-purification
    bait Wigge PA (1998) [37]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SPC34
  • YKR037C
    		•  The BioGRID Database Seperator Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
    Gene Ontology: [View List]
    Reconstituted Complex
    hit Shang C (2003) [35]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YRA1
  • SHE11
  • YDR381W
    		•  The BioGRID Database Seperator Nuclear protein that binds to RNA and to Mex67p, required for export of poly(A)+ mRNA from the nucleus; member of the REF (RNA and export factor binding proteins) family; another family member, Yra2p, can substitute for Yra1p function
    Gene Ontology: [View List]
    Affinity Capture-MS
    hit Krogan NJ (2006) [7079]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     NPY1
  • YGL067W
    		•  The BioGRID Database Seperator NADH diphosphatase (pyrophosphatase), hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes
    Gene Ontology: [View List]
    Affinity Capture-MS
    bait Krogan NJ (2006) [7079]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     MCM21
  • CTF5
  • YDR318W
    		•  The BioGRID Database Seperator Protein involved in minichromosome maintenance; component of the COMA complex (Ctf19p, Okp1p, Mcm21p, Ame1p) that bridges kinetochore subunits that are in contact with centromeric DNA and the subunits bound to microtubules
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CTF19
  • MCM18
  • YPL018W
    		•  The BioGRID Database Seperator Outer kinetochore protein, required for accurate mitotic chromosome segregation; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as a platform for kinetochore assembly
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     YKE2
  • GIM1
  • PFD6
  • YLR200W
    		•  The BioGRID Database Seperator Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CTK2
  • YJL006C
    		•  The BioGRID Database Seperator Beta subunit of C-terminal domain kinase I (CTDK-I), which phosphorylates the C-terminal repeated domain of the RNA polymerase II large subunit (Rpo21p) to affect both transcription and pre-mRNA 3' end processing; has similarity to cyclins
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     KAR3
  • OSR11
  • YPR141C
    		•  The BioGRID Database Seperator Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     MAD1
  • YGL086W
    		•  The BioGRID Database Seperator Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     MAD2
  • YJL030W
    		•  The BioGRID Database Seperator Component of the spindle-assembly checkpoint complex, which delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CIK1
  • YMR198W
    		•  The BioGRID Database Seperator Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p
    Gene Ontology: [View List]
    Synthetic Lethality
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CTF4
  • CHL15
  • POB1
  • YPR135W
    		•  The BioGRID Database Seperator Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     CTF3
  • CHL3
  • YLR381W
    		•  The BioGRID Database Seperator Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     APQ12
  • YIL040W
    		•  The BioGRID Database Seperator Protein involved in nucleocytoplasmic transport of mRNA
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SEC7
  • YDR170C
    		•  The BioGRID Database Seperator Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles
    Gene Ontology: [View List]
    Synthetic Lethality
    bait Davierwala AP (2005) [564]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     RFT1
  • YBL020W
    		•  The BioGRID Database Seperator Flippase, essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane; mutation is suppressed by expression human p53 protein
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     GIM4
  • PFD2
  • YEL003W
    		•  The BioGRID Database Seperator Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     RIM21
  • PAL2
  • YNL294C
    		•  The BioGRID Database Seperator Component of the RIM101 pathway, has a role in cell wall construction and alkaline pH response; has similarity to A. nidulans PalH
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     SST2
  • YLR452C
    		•  The BioGRID Database Seperator GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
     VPS71
  • SWC6
  • YML041C
    		•  The BioGRID Database Seperator Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
    Gene Ontology: [View List]
    Synthetic Growth Defect
    hit Montpetit B (2005) [49]
    The BioGRID Database Seperator
    The BioGRID Database Seperator
    The BioGRID Database Seperator
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